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Potential Use of a Pathogenic Yeast Pichia kluyveri FM012 for Degradation of Dichlorodiphenyltrichloroethane (DDT)

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Abstract

Many organochlorine pesticides (OCPs) are considerably high toxic, and have bioaccumulation potential and chronic adverse impact on both wildlife and human. This study focuses on the fate and metabolic degradation, which is the potential to be more efficient, economic, and safe compared to the aforementioned conventional methods. By these positive attributes, the present work then investigates the capability of newly isolated pathogenic yeast Pichia kluyveri FM012 for biodegradation of DDT in aquatic culture. Pichia kluyveri FM012 mycelia were cultured in a mineral liquid medium consisting of the solution of DDT (40 mg/l) with some experimental conditions such as the initial pH of the culture (5–8), agitation speed (0–150 rpm), and various carbon and nitrogen sources. The highest biodegradation of DDT by Pichia kluyveri FM012 was shown in the culture with pH 5 and 150 rpm agitation. Moreover, the use of glucose and yeast offers the best performance for the degradation compared to other carbon and nitrogen sources. The highest enzyme activity during the decolorization process was dioxygenase. Fourier-transform infrared spectroscopy (FTIR), UV-Vis spectrophotometer, and GC-MS profile showed that the transformation of DDT has occurred. The present DDE and DDD as metabolites of DDT were confirmed by GCMS at a retention time of 17.8 and 16.6 min. The outcomes of this study have several important implications for future practice, for instance in providing an alternative biodegradation agent to remove some organochlorine pollutants.

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Funding

This work was supported by the Deanship of Scientific Research at King Saud University through research group no. RG 1439-044 and by FRGS grant no. 4F813 of the Malaysian Ministry of Higher Education.

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Authors and Affiliations

  1. Department of Water and Environmental Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Ismalianto Isia

  2. Department of Environmental Engineering, Faculty of Engineering and Science, Curtin University, 98009, Miri, Sarawak, Malaysia

    Tony Hadibarata

  3. Research Unit for Clean Technology, Indonesian Institute of Sciences, Jalan Cisitu, Sangkuriang, Bandung, Jawa Barat, 40135, Indonesia

    Ajeng Arum Sari

  4. Botany and Microbiology Department, College of Sciences, King Saud University, Girls Campus, P.O. Box 22452, Riyadh, 11495, Saudi Arabia

    Dunia A. Al Farraj & Manal M. Al Khulaifi

  5. Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Mohamed Soliman Elshikh

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  1. Ismalianto Isia
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  2. Tony Hadibarata
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Correspondence to Tony Hadibarata.

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Isia, I., Hadibarata, T., Sari, A.A. et al. Potential Use of a Pathogenic Yeast Pichia kluyveri FM012 for Degradation of Dichlorodiphenyltrichloroethane (DDT). Water Air Soil Pollut 230, 221 (2019). https://doi.org/10.1007/s11270-019-4265-z

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