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Detoxification of Mercury by Bacteria Using Crude Glycerol from Biodiesel as a Carbon Source

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Abstract

Bacteria that harbor the mer operon in their genome are able to enzymatically reduce mercury (II) to the volatile form of mercury Hg (0). Detoxification of contaminated waste by using these bacteria may be an alternative to conventional methods for mercury removal. Residual glycerol from the biodiesel industry can be used as a carbon source to accelerate the process. This work shows for the first time the feasibility of using residual glycerol as a carbon source for Hg removal by bacteria prospected from contaminated environments. Eight bacterial isolates were able to remove mercury and degrade glycerol in mineral medium and residual glycerol. Mercury removal was monitored by atomic absorption spectroscopy and glycerol degradation by high performance liquid chromatography. The best results of mercury removal and glycerol degradation were obtained using isolates of Serratia marcescens M25C (85 and 100 %), Klebsiella pneumoniae PLB (90 and 100 %), Klebsiella oxytoca (90 and 100 %), and Arthrobacter sp. U3 (80 and 75 %), with addition of 0.5 g L−1 yeast extract. The Arthrobacter sp. U3 isolate is common in soils and has proven to be a promising candidate for environment applications due to its low pathogenicity and higher Hg removal and glycerol degradation rates.

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Acknowledgments

The authors wish to thank the Brazilian Bureau for Science and Technology (CNPq) and CAPES for the financial support for this research.

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Authors and Affiliations

  1. Fuels and Biofuels Biodeterioration Laboratory (LAB-BIO), Department of Microbiology, Immunology and Parasitology, Institute of Health Science, Federal University of Rio Grande do Sul, 500 Sarmento Leite St, Porto Alegre, RS, 90050-170, Brazil

    P. Giovanella, A. P. Costa & F. M. Bento

  2. Department of Soil Science, Federal University of Rio Grande do Sul, 7712 Bento Gonçalves Ave, Porto Alegre, RS, 91540-000, Brazil

    N. Schäffer & F. A. O. Camargo

  3. Department of Organic Chemistry, Institute of Chemistry, Federal University of Rio Grande do Sul, 9500 Bento Gonçalves Ave, Porto Alegre, RS, 91540-000, Brazil

    M. C. R. Peralba

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  1. P. Giovanella
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Giovanella, P., Costa, A.P., Schäffer, N. et al. Detoxification of Mercury by Bacteria Using Crude Glycerol from Biodiesel as a Carbon Source. Water Air Soil Pollut 226, 224 (2015). https://doi.org/10.1007/s11270-015-2480-9

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  • DOI: https://doi.org/10.1007/s11270-015-2480-9

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