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A Novel Bacterium That Degrades Aroclor-1254 and Its bphC Gene Encodes an Extradiol Aromatic Ring Cleavage Dioxygenase (EARCD)

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Abstract

The congener-specific degradation pattern of polychlorinated biphenyls (PCBs) in Aroclor 1254 was investigated using a novel bacterial strain, Stenotrophomonas sp. JSG1, and it was accelerated by the surfactant, β-cyclodextrin. In addition, 4-chorobenzoic acid (CBA) degradation was also confirmed by the estimation of CBA depletion rate, microbial growth, and release of free chloride ion in mineral medium. Metal ions such as Ni2+, Hg2+ Ba2+, Cu2+, and NaCl (>4 %) were found greatly influencing the PCB degradation. However, Ca2+, Mg2+, Fe2+, and Mn2+ have not shown any impact on biodegradation. The bphC gene, which encodes an extradiol aromatic ring cleavage dioxygenase was successfully amplified and cloned. Phylogeny-based pairwise alignment of nucleotide sequences suggested that the cloned gene belongs to the extradiol dioxygenase family, but it showed high diversity to the traditional bphC gene. Results of the present investigation revealed that the Stenotrophomonas sp. JSG1 is an effective novel bacterium, which can be used in the PCB remediation studies.

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Acknowledgments

This work has been financially supported by the ‘Council of Scientific & Industrial Research-Human resource Development group’ (CSIR-HRDG) and the ‘Department of Biotechnology’ (DBT), India, which we gratefully thank.

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Authors and Affiliations

  1. Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu, India

    Selvaraj Ganesh-Kumar, Karuppanan Kalimuthu & Solomon RobinsonDavid Jebakumar

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  1. Selvaraj Ganesh-Kumar
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  2. Karuppanan Kalimuthu
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  3. Solomon RobinsonDavid Jebakumar
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Correspondence to Solomon RobinsonDavid Jebakumar.

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Ganesh-Kumar, S., Kalimuthu, K. & Jebakumar, S.R. A Novel Bacterium That Degrades Aroclor-1254 and Its bphC Gene Encodes an Extradiol Aromatic Ring Cleavage Dioxygenase (EARCD). Water Air Soil Pollut 224, 1587 (2013). https://doi.org/10.1007/s11270-013-1587-0

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