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Quantitative Assessment of Effects of Cadmium on the Histological Structure of Poplar and Willow Leaves

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Abstract

Stem cuttings with homogenous diameter of Populus x euramericana (clone I-214) and Salix fragilis L. were grown in growth chamber in water culture method. After 45 days, the plants were treated with 10−7 and 10−5 M cadmium (Cd). As these species have different phytoextraction potentials, there is a need to analyze the level of Cd uptake, its translocation into aboveground organs, and changes in leaf structure. We analyzed micromorphological leaf characteristics: a fresh mass of the root, stem, and leaf, as well as a Cd concentration within them. Besides, we compared 23 micromorphological leaf blade quantitative traits of poplar and willow and monitored the structural changes induced by the intoxication of stem cuttings. Percent of Cd accumulation and translocation in plant organs varied between species. It depended on the level of Cd applied. When compared to the poplar clone, S. fragilis had a smaller leaf area and epidermal cells, thicker palisade tissue, smaller lumen of main vein vessels, and a higher percentage of main vein xylem. S. fragilis had more distinctive xeromorphic characteristics in the lamina structure. Increased concentrations of Cd led to significant structural changes, mainly in the main vein. When searching for valid parameters in assessing plant to be utilized in phytoremediation, it is necessary to take into consideration the interrelation of a large number of micromorphological parameters together with physiological and biochemical characteristics.

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Acknowledgments

A research presented in this paper was supported by the Ministry of Education and Science, Republic of Serbia, grant no. OI-173002 and III-043007.

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  1. Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, Serbia

    J. Luković, Lj. Merkulov, S. Pajević, L. Zorić, N. Nikolić, M. Borišev & D. Karanović

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  1. J. Luković
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Luković, J., Merkulov, L., Pajević, S. et al. Quantitative Assessment of Effects of Cadmium on the Histological Structure of Poplar and Willow Leaves. Water Air Soil Pollut 223, 2979–2993 (2012). https://doi.org/10.1007/s11270-012-1081-0

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