Abstract
In the present investigation, we report the immobilization of the enzyme tyrosinase on mesoporous silica material, i.e. MCM-41 to serve as a tool for the detection of phenol. The enzyme immobilized onto the MCM-41 matrix has shown to retain its activity and is quite stable. The immobilization of enzyme has been discussed, and the various factors that affect the loading of enzyme onto MCM-41 were studied and optimized. The applicability of tyrosinase-immobilized MCM-41 was then demonstrated for the detection of phenol. The lowest detectable concentration of phenol by tyrosinase-immobilized MCM-41 was observed to be 1 mg l−1. The factors influencing the detection of phenol were then studied in detail.
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Acknowledgements
The authors Priti A. Mangrulkar and Renu Yadav would also take the opportunity to sincerely acknowledge the Council of Scientific and Industrial Research India for granting the Senior Research Fellowship. The authors also gratefully acknowledge the Department of Chemistry, RTM Nagpur University and Director, NEERI for providing the research facilities and for constant support and encouragement.
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Mangrulkar, P.A., Yadav, R., Meshram, J.S. et al. Tyrosinase-Immobilized MCM-41 for the Detection of Phenol. Water Air Soil Pollut 223, 819–825 (2012). https://doi.org/10.1007/s11270-011-0904-8
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DOI: https://doi.org/10.1007/s11270-011-0904-8