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Influence of Different Substrates in Wetland Soils on Denitrification

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Abstract

Different substrates were evaluated to investigate their effect on nitrate removal and denitrifying bacterial community in soils obtained from wetland. Serial batch kinetic tests were conducted on soils obtained from wetland mixed with glucose and sawdust using KNO3 solution. Column tests were also conducted on soils obtained from wetland mixed with three different substrates (glucose, sawdust, and scoria coated with zero-valent iron) using KNO3 solution. For the batch tests, the nitrate removal efficiency for soil mixed with glucose was comparable to that for soil mixed with sawdust, but the nitrate removal rate for soil mixed with glucose (23.3 NO −3 -N mg/L-d) was approximately eight times higher than that for soil mixed with sawdust (2.8 NO −3 -N mg/L-d). For column tests among soil samples, nitrate removal efficiency was highest in soil mixed with glucose, which is an easily biodegradable carbon source. Removal efficiency increased with increasing incubation time for both soil samples with glucose and sawdust. A phylogenetic analysis based on nitrate reductase gene demonstrated that the different carbon sources affected both the diversity and compositions of the denitrifying bacterial in soil samples.

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Acknowledgement

This work was supported by Midcarrier Researcher Program through NRF grant funded by the MEST (No. R01-2007-20964-0). The opinions and findings reported in this paper are solely those of the authors, with no endorsement by the funding agency implied.

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Authors and Affiliations

  1. Department of Earth and Environmental Sciences, Korea University, Anam-dong, Seongbuk-gu, Seoul, 136-713, Republic of Korea

    Tran Trong Hien, Ho Young Jo & Seong-Taek Yun

  2. School of Civil, Environmental, and Architechtural Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul, 136-713, Republic of Korea

    Hee-Deung Park

  3. Institute of Geological Science, Vietnam Academy of Sciences and Technology, 84 Lane, Chua Lang Str., Dong Da District, Hanoi, Vietnam

    Tran Trong Hien & Nguyen Trung Minh

Authors
  1. Tran Trong Hien
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  2. Hee-Deung Park
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Correspondence to Ho Young Jo.

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Hien, T.T., Park, HD., Jo, H.Y. et al. Influence of Different Substrates in Wetland Soils on Denitrification. Water Air Soil Pollut 215, 549–560 (2011). https://doi.org/10.1007/s11270-010-0498-6

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  • DOI: https://doi.org/10.1007/s11270-010-0498-6

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