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MiR-339-5p inhibits replication of porcine reproductive and respiratory syndrome virus by targeting viral gene regions

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a variable virus, whose spread cannot be totally stopped by vaccination. PRRSV infection results in abortion and respiratory symptoms in pregnant pigs. One crucial component of the anti-viral infection strategy is microRNA (miRNA), a class of multifunctional small molecules. It is unknown whether miR-339-5p can specifically target the PRRSV gene and prevent the virus from replicating, despite the fact that miR-339-5p is markedly up-regulated during the PRRSV infection. In this pursuit, the present study revealed that the two PRRSV areas targeted by miR-339-5p were PRRSV nsp2-3378 to 3403 and PRRSV nsp2-3112 to 3133 using the miRanda program. Dual luciferase reporter assays showed that the miR-339-5p target region of the PRRSV gene sequence exhibited 100% homology and was highly conserved. Furthermore, the ability of miR-339-5p to target PRRSV gene areas was verified. It was found that the overexpression of miR-339-5p markedly reduced the PRRSV replication through PRRSV infection trials. The precursor sequence of ssc-miR-339-5p was amplified using the DNA of pig lung tissue as a template in order to create a fragment of 402 bp of porcine-derived miR-339-5p precursor sequence, which was then used to produce the eukaryotic expression plasmid of miR-339-5p. In conclusion, miR-339-5p can target the specific PRRSV gene areas and prevent PRRSV replication, offering fresh perspectives for the creation of medications that combat the PRRSV infection.

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No datasets were generated or analyzed during the current study.

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Funding

This study was supported by the Corps Key Area Science and Technology Research Program(2022AB012), the Young Innovative Talent Program of Shihezi University (CXPY202209, CXPY202211), and the Foundation for High-level Talents of Shihezi University (RCZK202043).

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Authors and Affiliations

  1. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China

    Cuifang Ye, Xinyan Cao, Jinliang Sheng, Yanming Sun & Yanbing Zhang

  2. Eighth Division, Animal Husbandry and Veterinary Workstation, Shihezi City, Xinjiang Production and Construction Corps, Shihezi, Xinjiang, China

    Guang Li & Wenbin Fang

Authors
  1. Cuifang Ye
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  2. Xinyan Cao
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  3. Jinliang Sheng
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  4. Yanming Sun
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  5. Guang Li
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  6. Wenbin Fang
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  7. Yanbing Zhang
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Contributions

CY: conceptualization, methodology, manuscript drafting, visualization. XC: conceptualization, visualization, methodology. YS and GL: methodology, visualization. JS: supervision. WF and YZ: conceptualization, supervision, writing—review & editing.

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Correspondence to Wenbin Fang or Yanbing Zhang.

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Edited by Zhen Fu.

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Ye, C., Cao, X., Sheng, J. et al. MiR-339-5p inhibits replication of porcine reproductive and respiratory syndrome virus by targeting viral gene regions. Virus Genes 60, 186–193 (2024). https://doi.org/10.1007/s11262-024-02059-6

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  • DOI: https://doi.org/10.1007/s11262-024-02059-6

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