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Genetic characteristics of a novel HIV-1 recombinant lineage (CRF103_01B) and its prevalence in northern China

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Abstract

During the routine surveillance of HIV-1 pretreatment drug resistance in Beijing, five men who have sex with men (MSM) and a woman were observed to get infected by newly identified CRF103_01B strain. To elucidate the genetic characteristics, the near full-length genome (NFLG) was obtained. Phylogenetic inference indicated that CRF103_01B NFLG was composed of six mosaic segments. Segments IV and V of CRF103_01B were located among the clusters subtype B and CRF01_AE (group 5), respectively. The CRF103_01B strain was deduced to originate from Beijing MSM population around 2002.3–2006.4 and continued to spread among MSM population at a low level, then to the general population via heterosexual contact in northern China. Molecular epidemiology surveillance of CRF103_01B should be reinforced.

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References

  1. Bbosa N, Kaleebu P, Ssemwanga D (2019) HIV subtype diversity worldwide. Curr Opin HIV AIDS 14:153–160. https://doi.org/10.1097/COH.0000000000000534

    Article  PubMed  Google Scholar 

  2. Robertson DL, Anderson JP, Bradac JA, Carr JK, Foley B, Funkhouser RK, Gao F, Hahn BH, Kalish ML, Kuiken C, Learn GH, Leitner T, McCutchan F, Osmanov S, Peeters M, Pieniazek D, Salminen M, Sharp PM, Wolinsky S, Korber B (2000) HIV-1 nomenclature proposal. Science 288:55–56. https://doi.org/10.1126/science.288.5463.55d

    Article  CAS  PubMed  Google Scholar 

  3. Yamaguchi J, Vallari A, McArthur C, Sthreshley L, Cloherty GA, Berg MG, Rodgers MA (2020) Complete genome sequence of CG-0018a-01 establishes HIV-1 subtype L. J Acquir Immune Defic Syndr 83:319–322. https://doi.org/10.1097/QAI.0000000000002246

    Article  PubMed  Google Scholar 

  4. Ramirez BC, Simon-Loriere E, Galetto R, Negroni M (2008) Implications of recombination for HIV diversity. Virus Res 134:64–73. https://doi.org/10.1016/j.virusres.2008.01.007

    Article  CAS  PubMed  Google Scholar 

  5. Fang G, Weiser B, Kuiken C, Philpott SM, Rowland-Jones S, Plummer F, Kimani J, Shi B, Kaul R, Bwayo J, Anzala O, Burger H (2004) Recombination following superinfection by HIV-1. AIDS 18:153–159. https://doi.org/10.1097/00002030-200401230-00003

    Article  CAS  PubMed  Google Scholar 

  6. Steain MC, Wang B, Dwyer DE, Saksena NK (2004) HIV-1 co-infection, superinfection and recombination. Sex Health 1:239–250. https://doi.org/10.1071/sh04024

    Article  PubMed  Google Scholar 

  7. Luan H, Han X, Yu X, An M, Zhang H, Zhao B, Xu J, Chu Z, Shang H (2017) Dual infection contributes to rapid disease progression in men who have sex with men in China. J Acquir Immune Defic Syndr 75:480–487. https://doi.org/10.1097/QAI.0000000000001420

    Article  PubMed  PubMed Central  Google Scholar 

  8. Wagner GA, Pacold ME, Kosakovsky Pond SL, Caballero G, Chaillon A, Rudolph AE, Morris SR, Little SJ, Richman DD, Smith DM (2014) Incidence and prevalence of intrasubtype HIV-1 dual infection in at-risk men in the United States. J Infect Dis 209:1032–1038. https://doi.org/10.1093/infdis/jit633

    Article  CAS  PubMed  Google Scholar 

  9. Liu Y, Su B, Zhang Y, Jia L, Li H, Li Z, Han J, Zhang T, Li T, Wu H, Wang X, Li J, Li L (2019) Onward transmission of multiple HIV-1 unique recombinant forms among men who have sex with Men in Beijing, China. J Acquir Immune Defic Syndr 81:1–4. https://doi.org/10.1097/QAI.0000000000001983

    Article  PubMed  Google Scholar 

  10. Li X, Li W, Zhong P, Fang K, Zhu K, Musa TH, Song Y, Du G, Gao R, Guo Y, Yan W, Xuan Y, Wei P (2016) Nationwide trends in molecular epidemiology of HIV-1 in China. AIDS Res Hum Retroviruses 32:851–859. https://doi.org/10.1089/AID.2016.0029

    Article  PubMed  Google Scholar 

  11. Hao J, Zheng S, Gan M, Dong A, Kang R, Li M, Zhao S, Hu J, Song C, Liao L, Feng Y, Shao Y, Ruan Y, Xing H (2021) Changing proportions of HIV-1 subtypes and transmitted drug resistance among newly diagnosed HIV/AIDS individuals—China, 2015 and 2018. China CDC Weekly 3:1133–1138. https://doi.org/10.46234/ccdcw2021.251

    Article  PubMed  PubMed Central  Google Scholar 

  12. He X, Xing H, Ruan Y, Hong K, Cheng C, Hu Y, Xin R, Wei J, Feng Y, Hsi JH, Takebe Y, Shao Y (2012) A comprehensive mapping of HIV-1 genotypes in various risk groups and regions across China based on a nationwide molecular epidemiologic survey. PLoS ONE 7:e47289. https://doi.org/10.1371/journal.pone.0047289

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Zhao S, Feng Y, Hu J, Li Y, Zuo Z, Yan J, Zhang J, Cao P, Xu W, Li F, Li Y, Liao L, Ruan Y, Shao Y, Xing H (2018) Prevalence of Transmitted HIV drug resistance in antiretroviral treatment naïve newly diagnosed individuals in China. Sci Rep 8:12273. https://doi.org/10.1038/s41598-018-29202-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Sun L, Jia L, Liu Y, Han J, Li J, Li H, Li L (2019) Multiple HIV-1 subtypes were found circulating in Shijingshan district of Beijing, China. AIDS Res Hum Retroviruses 35:494–499. https://doi.org/10.1089/AID.2018.0263

    Article  CAS  PubMed  Google Scholar 

  15. Ye J, Hao M, Xing H, Wang Y, Wang J, Feng Y, Xin R, Zeng J, Zhao S, Hao Y, Chen J, Ruan Y, Li X, Shao Y, Lu H (2020) Characterization of subtypes and transmitted drug resistance strains of HIV among Beijing residents between 2001–2016. PLoS ONE 15:e0230779. https://doi.org/10.1371/journal.pone.0230779

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Ye J, Hao M, Xing H, Zhang F, Wu H, Lv W, Jiang T, Wang Y, Wang J, Feng Y, Xin R, Zeng J, Zhao S, Hao Y, Chen J, Ruan Y, Li X, Shao Y, Lu H (2020) Transmitted HIV drug resistance among individuals with newly diagnosed HIV infection: a multicenter observational study. AIDS 34:609–619. https://doi.org/10.1097/QAD.0000000000002468

    Article  PubMed  Google Scholar 

  17. Hao M, Wang J, He S, Hao Y, Ye J, Xin R, Lu H (2019) Identification of a novel HIV-1 second-generation recombinant form (CRF01_AE/07_BC) in men who have sex with men in Beijing, China. AIDS Res Hum Retroviruses 35:500–504. https://doi.org/10.1089/AID.2018.0228

    Article  CAS  PubMed  Google Scholar 

  18. Yao Y, Zeng Y, Huang H, Li J, Li J, Xin R (2021) Characteristics of four novel recombinant strains from the backbone of CRF55_01B and CRF65_cpx in Beijing by near full-length genome. AIDS Res Hum Retroviruses 37:936–945. https://doi.org/10.1089/AID.2020.0255

    Article  CAS  PubMed  Google Scholar 

  19. Zhou J, Lu X, Feng Y, Li M, Zhu Y, Kang R, Zhou Z, Liu L, Cao Z, Ge Z, Ou W, Li K, Ruan Y, Liao L, Shao Y, Xing H (2020) Genome sequence of a novel HIV-1 circulating recombinant form (CRF103_01B) identified from Hebei Province, China. AIDS Res Hum Retroviruses 36:234–241. https://doi.org/10.1089/AID.2019.0196

    Article  CAS  PubMed  Google Scholar 

  20. Shi P, Chen Z, Meng J, Su M, Yang X, Fan W, Shi H, Gao Y, Lu X (2021) Molecular transmission networks and pre-treatment drug resistance among individuals with acute HIV-1 infection in Baoding, China. PLoS ONE 16:e0260670. https://doi.org/10.1371/journal.pone.0260670

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Zeng R, Ren D, Gong X, Wei M, Gao L, Yu A, Zhang D, Mi Y, Ma P (2020) HIV-1 Genetic Diversity and High Prevalence of Pretreatment Drug Resistance in Tianjin, China. AIDS Res Hum Retroviruses 36:852–861. https://doi.org/10.1089/AID.2020.0056

    Article  CAS  PubMed  Google Scholar 

  22. da Costa CP, Rodrigues JKF, de Morais VMS, de Andrade C, Neves PAF, Lima K (2020) HIV-1 subtype frequency in Northeast Brazil: a systematic review and meta-analysis. J Med Virol 92:3219–3229. https://doi.org/10.1002/jmv.25842

    Article  CAS  Google Scholar 

  23. He C, Han X, Zhang H, Jiang F, An M, Zhao B, Ding H, Zhang Z, Dong T, Shang H (2019) High polymorphism rates in well-known T cell epitopes restricted by protective HLA alleles during HIV infection are associated with rapid disease progression in early-infected MSM in China. Med Microbiol Immunol 208:239–251. https://doi.org/10.1007/s00430-019-00585-x

    Article  CAS  PubMed  Google Scholar 

  24. Song H, Ou W, Feng Y, Zhang J, Li F, Hu J, Peng H, Xing H, Ma L, Tan Q, Li D, Wang L, Wu B, Shao Y (2019) Disparate impact on CD4 T cell count by two distinct HIV-1 phylogenetic clusters from the same clade. Proc Natl Acad Sci USA 116:239–244. https://doi.org/10.1073/pnas.1814714116

    Article  CAS  PubMed  Google Scholar 

  25. Cui H, Geng W, Sun H, Han X, An M, Jiang Y, Zhang Z, Chen Z, Xu J, Hu Q, Zhao B, Zhou B, Shang H (2019) Rapid CD4+ T-cell decline is associated with coreceptor switch among MSM primarily infected with HIV-1 CRF01_AE in Northeast China. AIDS 33:13–22. https://doi.org/10.1097/QAD.0000000000001981

    Article  PubMed  Google Scholar 

  26. Ge Z, Feng Y, Li K, Lv B, Zaongo SD, Sun J, Liang Y, Liu D, Xing H, Wei M, Ma P, Shao Y (2021) CRF01_AE and CRF01_AE cluster 4 are associated with poor immune recovery in Chinese patients under combination antiretroviral therapy. Clin Infect Dis 72:1799–1809. https://doi.org/10.1093/cid/ciaa380

    Article  CAS  PubMed  Google Scholar 

  27. Lengauer T, Sander O, Sierra S, Thielen A, Kaiser R (2007) Bioinformatics prediction of HIV coreceptor usage. Nat Biotechnol 25:1407–1410. https://doi.org/10.1038/nbt1371

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thank a lot for the individuals recruited for this study and the staff for the first diagnosis of the cases.

Funding

This study was sponsored by Beijing Natural Science Foundation (grant number: 7202074) and Capital's Funds for Health Improvement and Research (grant number: CFH2022-1G-3015).

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Author notes

  1. Man Dai and Jia Li have contributed equally to this work.

Authors and Affiliations

  1. China Medical University, Shenyang, 110122, China

    Man Dai

  2. Beijing Center for Disease Prevention and Control, Beijing, 100013, China

    Man Dai, Jia Li, Jie Li, Hongyan Lu, Chun Huang & Ruolei Xin

  3. Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China

    Shiyun Lv

  4. The Fifth Medical Center of PLA General Hospital, Beijing, 100039, China

    Huihuang Huang

Authors
  1. Man Dai
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  2. Jia Li
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Contributions

RLX and HHH conceived and designed the study. HHH and JL† collected the samples. MD, JL†, and SYL performed the experiments. JL, HYL, and CH analyzed the data. Writing of the original draft was done by Man Dai. Reviewing and editing of the manuscript were done by RLX. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Huihuang Huang or Ruolei Xin.

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Conflict of interest

The authors declare no conflict of interest. The funders had no role in the design of the study, in the recruitment or collection, analyses or interpretation of the data, in the writing of the manuscript, or in the decision to publish the results.

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Dai, M., Li, J., Li, J. et al. Genetic characteristics of a novel HIV-1 recombinant lineage (CRF103_01B) and its prevalence in northern China. Virus Genes 59, 643–650 (2023). https://doi.org/10.1007/s11262-023-01994-0

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  • DOI: https://doi.org/10.1007/s11262-023-01994-0

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