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Anguillid herpesvirus 1 (AngHV) ORF95 encodes a late, structural envelope protein

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Abstract

Anguillid herpesvirus 1 (AngHV) is one of the vital pathogenic agents found in the wild and cultured eel populations, which has brought significant losses to eel culture industry in China. In this study, AngHV ORF95 was characterized. Bioinformatics analysis showed that ORF95 putatively encodes a structural protein that is homologous to hemagglutinin-esterase (HE) protein of infectious salmon anemia virus (ISAV). Temporal transcription and expression analysis indicated that ORF95 is a viral late gene. Subcellular localization analysis revealed that ORF95 was predominantly localized in the cytoplasm. Further, western blot analysis indicated that ORF95 is a structural protein of virion envelope. These results provide a novel basis to make further efforts to clarify the function of ORF95 in the process of AngHV infection and the possibility to use ORF95 as antigen to develop AngHV subunit vaccine.

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References

  1. Jakob E, Neuhaus H, Steinhagen D, Luckhardt B, Hanel R (2009) Monitoring of Herpesvirus anguillae (HVA) infections in European eel, Anguilla anguilla (L.), in northern Germany. J Fish Dis 32:557–561. https://doi.org/10.1111/j.1365-2761.2009.01009.x

    Article  CAS  PubMed  Google Scholar 

  2. Kobayashi T, Miyazaki T (1997) Characterization and Pathogenicity of a Herpesvirus Isolated from Cutaneous Lesion in Japanese Eel, Anguilla japonica. Fish Pathology 32:89–95. https://doi.org/10.3147/jsfp.32.89

    Article  Google Scholar 

  3. Davidse A, Haenen OLM, Dijkstra SG, Wellenberg GJ, Nieuwstadt (1999) First isolation of herpesvirus of eel (Herpesvirus anguillae) in diseased European eel (Anguilla anguilla L) in Europe. Bull Eur Assoc Fish Pathol. https://doi.org/10.1515/BOT.1999.012

    Article  Google Scholar 

  4. van Ginneken V, Haenen O, Coldenhoff K, Willemze R, van den Thillart G (2004) Presence of eel viruses in eel species from various geographic regions. Bull Eur Assoc Fish Pathol 24:268–272

    Google Scholar 

  5. Chang PH, Pan YH, Wu CM, Kuo ST, Chung HY (2002) Isolation and molecular characterization of herpesvirus from cultured European eels Anguilla anguilla in Taiwan. Dis Aquat Organ 50:111–118. https://doi.org/10.3354/dao050111

    Article  CAS  PubMed  Google Scholar 

  6. Rijsewijk F, Pritz-Verschuren S, Kerkhoff S, Botter A, Willemsen M, van Nieuwstadt T, Haenen O (2005) Development of a polymerase chain reaction for the detection of Anguillid herpesvirus DNA in eels based on the herpesvirus DNA polymerase gene. J Virol Methods 124:87–94. https://doi.org/10.1016/j.jviromet.2004.11.007

    Article  CAS  PubMed  Google Scholar 

  7. Waltzek TB, Kelley GO, Alfaro ME, Kurobe T, Davison AJ, Hedrick RP (2009) Phylogenetic relationships in the family Alloherpesviridae. Dis Aquat Organ 84:179–194. https://doi.org/10.3354/dao02023

    Article  CAS  PubMed  Google Scholar 

  8. Sano M, Fukuda H, Sano T (1990) Isolation and characterization of a new herpesvirus from eel. Pathol Mar Sci. https://doi.org/10.1016/B978-0-12-550755-4.50008-2

    Article  Google Scholar 

  9. Haenen OLM, Dijkstra SG, Tulden DA, Wellenberg GJ (2002) Herpesvirus anguillae (HVA) isolations from disease outbreaks in cultured European eel, Anguilla anguilla in The Netherlands since 1996. Bull Eur Assoc Fish Pathol. https://doi.org/10.1051/kmae:2002079

    Article  Google Scholar 

  10. Ge JQ, Yang JX, Gong H, Lin TL (2014) Isolation and identification of a herpesvirus from cultured European eels Anguilla anguilla in China. J Fish China 38:1579–1583

    CAS  Google Scholar 

  11. van Beurden SJ, Peeters BP, Rottier PJ, Davison AJ, Engelsma MY (2013) Genome-wide gene expression analysis of anguillid herpesvirus 1. BMC Genomics 14:83. https://doi.org/10.1186/1471-2164-14-83

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. van Beurden SJ, Bossers A, Voorbergen-Laarman MH, Haenen OL, Peters S, Abma-Henkens MH, Peeters BP et al (2010) Complete genome sequence and taxonomic position of anguillid herpesvirus 1. J Gen Virol 91:880–887. https://doi.org/10.1099/vir.0.016261-0

    Article  CAS  PubMed  Google Scholar 

  13. van Beurden SJ, Leroy B, Wattiez R, Haenen OL, Boeren S, Vervoort JJ, Peeters BP et al (2011) Identification and localization of the structural proteins of anguillid herpesvirus 1. Vet Res 42:105. https://doi.org/10.1186/1297-9716-42-105

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. McGeoch DJ, Rixon FJ, Davison AJ (2006) Topics in herpesvirus genomics and evolution. Virus Res 117:90–104. https://doi.org/10.1016/j.virusres.2006.01.002

    Article  CAS  PubMed  Google Scholar 

  15. Rechenchoski DZ, Faccin-Galhardi LC, Linhares R, Nozawa C (2017) Herpesvirus: an underestimated virus. Folia Microbiol 62:151–156. https://doi.org/10.1007/s12223-016-0482-7

    Article  CAS  Google Scholar 

  16. Davison AJ (2002) Evolution of the herpesviruses. Vet Microbiol 86:69–88. https://doi.org/10.1016/s0378-1135(01)00492-8

    Article  CAS  PubMed  Google Scholar 

  17. Rimstad E, Mjaaland S, Snow M, Mikalsen AB, Cunningham CO (2001) Characterization of the infectious salmon anemia virus genomic segment that encodes the putative hemagglutinin. J Virol 75:5352–5356. https://doi.org/10.1128/JVI.75.11.5352-5356.2001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Falk K, Aspehaug V, Vlasak R, Endresen C (2004) Identification and characterization of viral structural proteins of infectious salmon anemia virus. J Virol 78:3063–3071. https://doi.org/10.1128/jvi.78.6.3063-3071.2004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Muller A, Markussen T, Drablos F, Gjoen T, Jorgensen TO, Solem ST, Mjaaland S (2010) Structural and functional analysis of the hemagglutinin-esterase of infectious salmon anaemia virus. Virus Res 151:131–141. https://doi.org/10.1016/j.virusres.2010.03.020

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Li YJ, Ge JQ, Song TY, Lin TL (2012) Prokaryotic Expression of anguillid herpesvirus ORF95 protein. Fujian J Agric Sci 27:787–791

    CAS  Google Scholar 

  21. Jameson BA, Wolf H (1988) The antigenic index: a novel algorithm for predicting antigenic determinants. Bioinformatics 4(1):181–186

    Article  CAS  Google Scholar 

  22. Tandon R, Mocarski ES (2012) Viral and host control of cytomegalovirus maturation. Trends Microbiol 20:392–401. https://doi.org/10.1016/j.tim.2012.04.008

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Wu H, Kropff B, Mach M, Britt WJ (2020) Human cytomegalovirus envelope protein gpUL132 regulates infectious virus production through formation of the viral assembly compartment. MBio. https://doi.org/10.1128/mBio.02044-20

    Article  PubMed  PubMed Central  Google Scholar 

  24. D’Costa SM, Yao H, Bilimoria SL (2001) Transcription and temporal cascade in chilo iridescent virus infected cells. Arch Virol 146:2165–2178. https://doi.org/10.1007/s007050170027

    Article  CAS  PubMed  Google Scholar 

  25. Knipe DM, Cliffe A (2008) Chromatin control of herpes simplex virus lytic and latent infection. Nat Rev Microbiol 6:211–221. https://doi.org/10.1038/nrmicro1794

    Article  CAS  PubMed  Google Scholar 

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Funding

This work was supported by Public-Interest Scientific Institution Basal Research Fund of Fujian Province (2018R1019-4, 2019R1027-4), National Natural Science Foundation of China (31101933), and Natural Science Foundation of Fujian Province (2019J01109).

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Authors and Affiliations

  1. Institute of Biotechnology, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, 350003, China

    Xi Chen, Jin-Xian Yang, Ying-Ying Li, Tie-Ying Song & Jun-Qing Ge

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  1. Xi Chen
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  2. Jin-Xian Yang
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  3. Ying-Ying Li
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  4. Tie-Ying Song
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  5. Jun-Qing Ge
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Contributions

XC and J-QG conceived and designed the experiments. XC, J-XY, Y-YL collected the samples. XC and J-XY performed the experiments. XC and Y-YL analyzed the data. XC wrote the initial draft of the manuscript. T-YS and J-QG revised the manuscript.

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Correspondence to Jun-Qing Ge.

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This research did not involve any animal and/or human participants.

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Edited by Juergen A Richt.

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Chen, X., Yang, JX., Li, YY. et al. Anguillid herpesvirus 1 (AngHV) ORF95 encodes a late, structural envelope protein. Virus Genes 57, 280–283 (2021). https://doi.org/10.1007/s11262-021-01836-x

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  • DOI: https://doi.org/10.1007/s11262-021-01836-x

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