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PK15 cell line stably overexpressing IL2 enhances PCV2 replication

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Abstract

Porcine circovirus type 2 (PCV2) is the primary agent responsible for porcine circovirus-associated diseases (PCVADs), which is acknowledged as one of the most economically important diseases for the swine industry worldwide. Currently, the development of PCV2 vaccine against PCVADs and for other applications require large amounts of viral particles. The low propagation rate of PCV2 in vitro limits vaccine production. Previous studies showed that a cell line transfected with the porcine interleukin (IL)-2 gene gave higher PCV2 yield in vitro. However, transient transfection may become less effective and unstable after serial generations. In this work, we constructed a PK15 cell line with stable expression of porcine IL2 by lentivirus transfection. The results demonstrated that the transgenic cell line stably expressed IL2 protein significantly enhanced PCV2 replication. Thus, the transgenic PK15 cell line could be a promising cell line for vaccine production.

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References

  1. Liu J, Zhang X, Ma C, Jiang P, Yun S (2017) Hsp90 inhibitor reduces porcine circovirus 2 replication in the porcine monocytic line 3D4/31. Virus Genes 53:95–99

    Article  CAS  Google Scholar 

  2. Liu J, Zhang X, Ma C, You J, Dong M, Yun S, Jiang P (2016) Heat shock protein 90 is essential for replication of porcine circovirus type 2 in PK-15 cells. Virus Res 224:29–37

    Article  CAS  Google Scholar 

  3. Meng XJ (2013) Porcine circovirus type 2 (PCV2): pathogenesis and interaction with the immune system. Annu Rev Anim Biosci 1:43–64

    Article  Google Scholar 

  4. Karuppannan AK, Opriessnig T (2017) Porcine circovirus type 2 (PCV2) vaccines in the context of current molecular epidemiology. Viruses 9:99

    Article  Google Scholar 

  5. Afghah Z, Webb B, Meng XJ, Ramamoorthy S (2017) Ten years of PCV2 vaccines and vaccination: is eradication a possibility? Vet Microbiol 206:21–28

    Article  CAS  Google Scholar 

  6. Tischer I, Peters D, Rasch R, Pociuli S (1987) Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch Virol 96:39–57

    Article  CAS  Google Scholar 

  7. Yang X, Chen F, Cao Y, Pang D, Ouyang H, Ren L (2013) Comparative analysis of different methods to enhance porcine circovirus 2 replication. J Virol Methods 187:368–371

    Article  CAS  Google Scholar 

  8. Meerts P, Misinzo G, Nauwynck HJ (2005) Enhancement of porcine circovirus 2 replication in porcine cell lines by IFN-γ before and after treatment and by IFN-α after treatment. J Interferon Cytokine Res 25:684–693

    Article  CAS  Google Scholar 

  9. Mutthi P, Theerawatanasirikul S, Roytrakul S, Paemanee A, Lekcharoensuk C, Hansoongnern P, Petcharat N, Thangthamniyom N, Lekcharoensuk P (2018) Interferon gamma induces cellular protein alteration and increases replication of porcine circovirus type 2 in PK-15 cells. Arch Virol 163:2947–2957

    Article  CAS  Google Scholar 

  10. Zhu Y, Lau A, Lau J, Jia Q, Karuppannan AK, Kwang J (2007) Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line. Virology 369:423–430

    Article  CAS  Google Scholar 

  11. Ma T, Ouyang T, Ouyang H, Chen F, Peng Z, Chen X, Pang D, Ren L (2017) Porcine circovirus 2 proliferation can be enhanced by stably expressing porcine IL-2 gene in PK-15 cell. Virus Res 227:143–149

    Article  CAS  Google Scholar 

  12. Sachdeva G, D’Costa J, Cho JE, Kachapati K, Choudhry V, Arya SK (2007) Chimeric HIV‐1 and HIV‐2 lentiviral vectors with added safety insurance. J Med Virol 79:118–126

    Article  CAS  Google Scholar 

  13. Dai C, Sun L, Yu L, Zhu G, Wu S, Bao W (2016) Effects of porcine MyD88 knockdown on the expression of TLR4 pathway-related genes and proinflammatory cytokines. Biosci Rep 36:e00409

    Article  CAS  Google Scholar 

  14. Feng Z, Jiang P, Wang X, Li Y, Jiang W (2008) Adenovirus-mediated shRNA interference against porcine circovirus type 2 replication both in vitro and in vivo. Antiviral Res 77:186–194

    Article  CAS  Google Scholar 

  15. Opriessnig T, Meng XJ, Halbur PG (2007) Porcine circovirus type 2–associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Investig 19:591–615

    Article  Google Scholar 

  16. Gan F, Zhang Z, Hu Z, Hesketh J, Xue H, Chen X, Hao S, Huang Y, Cole EP, Parveen F, Huang K (2015) Ochratoxin A promotes porcine circovirus type 2 replication in vitro and in vivo. Free Radic Biol Med 80:33–47

    Article  CAS  Google Scholar 

  17. Qian G, Liu D, Hu J, Gan F, Hou L, Chen X, Huang K (2017) Ochratoxin A-induced autophagy in vitro and in vivo promotes porcine circovirus type 2 replication. Cell Death Dis 8:e2909

    Article  CAS  Google Scholar 

  18. Dignass AU, Podolsky DK (1996) Interleukin 2 modulates intestinal epithelial cell functionin vitro. Exp Cell Res 225:422–429

    Article  CAS  Google Scholar 

  19. Todd B, Pope JH, Georghiou P (1991) Interleukin-2 enhances production in 24 hours of infectious human immunodeficiency virus type 1 in vitro by naturally infected mononuclear cells from seropositive donors. Arch Virol 121:227–232

    Article  CAS  Google Scholar 

  20. Yan Q, Lan YH, Huang YX, Fan RS, Liu L, Song SP, Li YG (2016) Hepatitis B virus replication is upregulated in proliferated peripheral blood lymphocytes. Mol Med Rep 13:3581–3587

    Article  CAS  Google Scholar 

  21. O’Loughlin EV, Pang GP, Noltorp R, Koina C, Batey R, Clancy R (2001) Interleukin 2 modulates ion secretion and cell proliferation in cultured human small intestinal enterocytes. Gut 49:636–643

    Article  CAS  Google Scholar 

  22. Cheung AK, Bolin SR (2002) Kinetics of porcine circovirus type 2 replication. Arch Virol 147:43–58

    Article  CAS  Google Scholar 

  23. Meerts P, Misinzo G, McNeilly F, Nauwynck HJ (2005) Replication kinetics of different porcine circovirus 2 strains in PK-15 cells, fetal cardiomyocytes and macrophages. Arch Virol 150:427–441

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No: 31502063). We thank all the anonymous reviewers for their helpful suggestions on the quality improvement of our paper.

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Authors and Affiliations

  1. Department of Comparative Medicine, Jinling Hospital, Nanjing, People’s Republic of China

    Xuliang Zhang, Chang Ma, Jinwei You, Min Dong, Shifeng Yun & Jie Liu

  2. Clinical School of Medical College of Nanjing University, Nanjing, People’s Republic of China

    Shifeng Yun

  3. The Third Outpatient Department, Jinling Hospital, Nanjing, People’s Republic of China

    Yingfeng Zhao

Authors
  1. Xuliang Zhang
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  2. Yingfeng Zhao
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  3. Chang Ma
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  4. Jinwei You
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  5. Min Dong
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  6. Shifeng Yun
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  7. Jie Liu
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Contributions

Designed the study: JL. Wrote the manuscript: XZ. Performed the experiments and analyzed the data: XZ, YZ, CM, JY, and MD. Coordinated and facilitated the project: SY and JL. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Shifeng Yun or Jie Liu.

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All authors declare that no conflicts of interest exist.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Edited by Seung-Kook Choi.

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Zhang, X., Zhao, Y., Ma, C. et al. PK15 cell line stably overexpressing IL2 enhances PCV2 replication. Virus Genes 57, 111–116 (2021). https://doi.org/10.1007/s11262-020-01819-4

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  • DOI: https://doi.org/10.1007/s11262-020-01819-4

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