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Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India

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Abstract

Orf (contagious ecthyma) is an exanthematic disease caused by a parapoxvirus and occurs primarily in sheep and goats with zoonotic implications. In the present investigation, an orf outbreak in the Muzzaffarnagari sheep flock at the Central Institute for Research on Goats (CIRG), Makhdoom, Mathura, Uttar Pradesh, India, was investigated. Primary goat testes cell culture was used for isolation of the orf virus (ORFV) for the first time. The identity of the virus was confirmed by amplification and sequence analysis of the major envelope glycoprotein (B2L) gene and named ORFV/sheep/India/2012/CIRG. On phylogenetic analysis of B2L protein gene, it clustered with the ORFV strains from China suggesting distinct ORFV strains are circulating in India. On comparison of nucleotide and deduced amino acid sequence analysis (n = 63), a unique 126S residue was observed in ORFV/sheep/India/2012/CIRG. On further sequence analysis (B2L) of different ORFV strains (n = 63), some conserved amino acid residues were identified as host-specific (sheep, human, camel, takin, and musk ox) and have been summarized.

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Acknowledgments

Authors wish to thank to the Director Dr. S. K. Agarwal for administrative help in purchase of consumable. Former Director, Dr. D. Swaroop for arranging necessary funds on priority basis for the cell culture and virus isolation laboratory is highly acknowledged. This study was supported in part by Institute (CIRG) project (IXX08031) to N. Kumar.

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Authors and Affiliations

  1. Virology Laboratory, Division of Animal Health, Central Institute for Research on Goats, Indian Council of Agricultural Research, Makhdoom, P.O.-Farah, Mathura, UP, 281122, India

    Naveen Kumar, Kundan K. Chaubey, Shoor V. Singh, Sorabh Gupta, Dinesh K. Sharma & A. K. Mishra

  2. Division of Animal Genetics and Breeding, Central Institute for Research on Goats, Indian Council of Agricultural Research Makhdoom, P.O.-Farah, Mathura, 281122, UP, India

    Manoj K. Singh

  3. Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, 37996, USA

    Shalini Sharma

  4. Center for Wildlife Health, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, 37996, USA

    Ashutosh Wadhwa

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  1. Naveen Kumar
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Correspondence to Naveen Kumar.

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Kumar, N., Wadhwa, A., Chaubey, K.K. et al. Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India. Virus Genes 48, 312–319 (2014). https://doi.org/10.1007/s11262-013-1025-9

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  • DOI: https://doi.org/10.1007/s11262-013-1025-9

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