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Pistachio (Pistacia vera L.) is a new natural host of Hop stunt viroid

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Abstract

Besides hop, Hop stunt viroid (HpSVd) infects many woody species including grapevine, citrus, peach, plum, apricot, almond, pomegranate, mulberry and jujube. Here, we report the first detection of HpSVd in pistachio (Pistacia vera L.). Samples corresponding to 16 pistachio cultivars were obtained from a nearby almond collection. From these samples, low molecular weight RNAs were extracted for double polyacrylamide gel electrophoresis, northern-blot analysis and reverse transcription polymerase chain reaction assays. HpSVd was detected in 4 of the 16 pistachio cultivars in the first year and in 6 in the second, being also detected in the almond collection. Examination of the nucleotide sequences of pistachio and almond isolates revealed 13 new sequence variants. Sequences from pistachio shared 92–96 % similarity with the first reported HpSVd sequence (GenBank X00009), and multiple alignment and phylogenetic analyses showed that one pistachio isolate (HpSVdPis67Jabari) clustered with the plum group, whereas all the others clustered with the hop, and the recombinants plum-citrus and plum-Hop/cit3 groups. By identifying pistachio as a new natural host, we confirm that HpSVd is an ubiquitous and genetically variable viroid that infects many different fruit trees cultivated worldwide.

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Acknowledgments

We are grateful to R. Flores (CSIC-Universidad Politécnica de Valencia, Spain) for helpful discussions, critical reading of the manuscript and the plasmids kindly supplied. This work was partly supported by the collaborative Hispano-Tunisian program from the AECID (Agencia Espanola de cooperación internacional y Desarrollo) and the Tunisian Ministry of Higher Education, Scientific Research and Technology.

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Authors and Affiliations

  1. Laboratoire des Biotechnologies Végétales Appliquées à l’Amélioration des Cultures, Faculté des Sciences de Sfax, Université de Sfax, BP 1171, 3000, Sfax, Tunisia

    Amine Elleuch & Noureddine Drira

  2. Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie Faculté des Sciences de Tunis, Campus Universitaire El Manar, 2092, Tunis, Tunisia

    Imen Hamdi & Hatem Fkahfakh

  3. Labratoire de Bioconversion des enzymes Ecole Nationale des Ingénieurs de Sfax, Sfax, Tunisia

    Olfa Ellouze

  4. Laboratoire d’Amélioration de la Productivité de l’Olivier et des Arbres Fruitiers, Institut de l’Olivier, BP 1087, 3003, Sfax, Tunisia

    Mohamed Ghrab

  5. Centre Régional de Recherche Agronomique a Sidi Bouzid, Sidi Bouzid, Tunisia

    Imen Hamdi

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  1. Amine Elleuch
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  2. Imen Hamdi
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  3. Olfa Ellouze
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  4. Mohamed Ghrab
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  5. Hatem Fkahfakh
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  6. Noureddine Drira
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Correspondence to Amine Elleuch.

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Elleuch, A., Hamdi, I., Ellouze, O. et al. Pistachio (Pistacia vera L.) is a new natural host of Hop stunt viroid . Virus Genes 47, 330–337 (2013). https://doi.org/10.1007/s11262-013-0929-8

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  • DOI: https://doi.org/10.1007/s11262-013-0929-8

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