Abstract
Megalocytiviruses, which belong to the family Iridoviridae, are among the most harmful viruses to cultured fishes. Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the Megalocytivirus genus. The genome of ISKNV was sequenced and annotated by He et al. (Virology 291:126–139, 2001) in 2001 and re-annotated by Eaton et al. (Virol. J. 4:11, 2007) in 2007. The ORF092R of ISKNV was identified in the annotations of He et al. but was not reported by Eaton et al. In this study, by using Northern-blot and RACE assays, we identified the ORF092R transcript, indicating that ORF092R was indeed transcribed in the ISKNV-infected cells. Immunofluorescence and Western-blot showed that VP92R protein was expressed in the ISKNV-infected cells, and the molecular mass of VP92R is consistent with the theoretical one. Sequence comparison demonstrated that the VP92R orthologous protein is present in large yellow croaker iridovirus (LYCIV), but not in rock bream iridovirus (RBIV) or orange-spotted grouper iridovirus (OSGIV). Therefore, VP92R may have specific functions during ISKNV pathogenesis and could be a subject for studying the differences between megalocytiviruses.
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Acknowledgments
This research was supported by the National Natural Science Foundation of China under Grant Nos. 30325035 and U0631008, the National Basic Research Program of China under Grant No. 2006CB101802, the National High Technology Research and Development Program of China (863 Program) under Grant Nos. 2006AA09Z445 and 2006AA100309, the Guangdong Province Natural Science Foundation under grant No. 20023002, and the Science and Technology Bureau of Guangdong Province.
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Xiaopeng Xu and Qingxia Lu both contributed equally to this study.
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Xu, X., Lu, Q., Wang, R. et al. Identification of the VP92R gene from infectious spleen and kidney necrosis virus. Virus Genes 41, 210–217 (2010). https://doi.org/10.1007/s11262-010-0502-7
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DOI: https://doi.org/10.1007/s11262-010-0502-7