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Identification and characterization of a novel thymidylate synthase from deep-sea thermophilic bacteriophage Geobacillus virus E2

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Abstract

Thymidylate synthase (TS) is essential for de novo synthesis of dTMP and is a key enzyme involved in DNA synthesis and transcriptional regulation of organisms. Due to their biologic importance, TSs have been intensively studied. In this investigation, a thermostable TS was identified from a deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2). It was demonstrated that GVE2-TS was highly homologous to known TSs and contained five characteristic conserved domains. The temporal analyses by Northern and Western blots revealed that the GVE2-TS was transcribed and expressed early after Geobacillus virus E2 infection, identifying it as a viral early gene. As shown by gel mobility shift assays, the recombinant GVE2-TS protein had the capacity to bind its own mRNA. Our study presented the first report on thymidylate synthase from deep-sea thermophilic bacteriophage.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (40576076), the China Ocean Mineral Resources R&D Association (DYXM-115-02-2-15), and Hi-Tech Research and Development Program of China (863 program of China) (2007AA091407).

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Authors and Affiliations

  1. School of Life Sciences, Xiamen University, Xiamen, 361005, People’s Republic of China

    Yiqian Wang

  2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, 178 Daxue Rd., Xiamen, 361005, People’s Republic of China

    Yiqian Wang & Xiaobo Zhang

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  1. Yiqian Wang
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  2. Xiaobo Zhang
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Correspondence to Xiaobo Zhang.

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Wang, Y., Zhang, X. Identification and characterization of a novel thymidylate synthase from deep-sea thermophilic bacteriophage Geobacillus virus E2. Virus Genes 37, 218–224 (2008). https://doi.org/10.1007/s11262-008-0258-5

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  • DOI: https://doi.org/10.1007/s11262-008-0258-5

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