Skip to main content

Advertisement

SpringerLink
Log in

Analysis of immunogenicity of minor envelope protein GP3 of porcine reproductive and respiratory syndrome virus in mice

  • Original Article
  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically significant viral diseases in swine industry. Though the minor envelope protein GP3 is associated with protective immunity, its immunogenicity and protective mechanism are poorly known. In this study, two recombinant adenoviruses, rAd-GP3 expressing complete GP3 and rAd-tGP3 expressing truncated GP3 in which aa2-64 were deleted, were constructed and the immunogenicity were tested in a mouse model. Four groups of BALB/c mice were immunized subcutaneously twice at 2-week internals with the recombinants rAd-GP3 and rAd-tGP3 or with wild type adenovirus (wtAd) and PBS as control. The results showed that the mice immunized with recombinant adenoviruses developed PRRSV-specific neutralizing antibodies and cellular immune response, including T-cell proliferation responses and cytotoxic T responses, by 2 weeks post-primary immunization. Moreover, the levels of immune responses of mice immunized with rAd-tGP3 were significantly higher than that of mice with rAd-GP3. It indicated that the first 64aa fragment of GP3 might affect the conformation of the antigen structures of GP3 protein. GP3 protein should be one of candidate molecules for developing a new safer effective vaccine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Reference

  1. D. Cavanagh, Arch. Virol. 142, 629 (1997)

    CAS  PubMed  Google Scholar 

  2. J.J.M. Meulenberg, M. Hulst, E.J. de Meijer, P.L.J. Moonen, B.A. Petersen-Den, E.P. de Kluyver, G. Wensvoort, R.J.M. Moormann, Virology 192, 62 (1993)

    Article  CAS  Google Scholar 

  3. W.H. Wu, Y. Fang, R. Farwell, M. Steffen-Bien, R.R. Rowland, J. Christopher-Hennings, E.A. Nelson, Virology 287, 183 (2001)

    Article  CAS  Google Scholar 

  4. K.K. Conzelmann, N. Visser, P. van Woensel, H.J. Thiel, Virology 193, 329 (1993)

    Article  CAS  Google Scholar 

  5. E.J. Snijder, J.J.M. Meulenberg, J. Gen. Virol. 79, 961 (1998)

    Article  CAS  Google Scholar 

  6. H. Mardassi, B. Massie, S. Dea, Virology 221, 98 (1996)

    Article  CAS  Google Scholar 

  7. E.M. Bautista, J.J.M. Meulenberg, C.S. Choi, T.W. Molitor, Arch. Virol. 141, 1357 (1996)

    Article  CAS  Google Scholar 

  8. J.J.M. Meulenberg, B.A. Petersen-Den, Virology 225, 44 (1996)

    Article  CAS  Google Scholar 

  9. A.P. van Nieuwstadt, J.J.M. Meulenberg, A. van Essen-Zandbergen, B.A. Petersen-Den, R.J. Bende, R.J.M. Moorman, G. Wensvoort, J. Virol. 70, 4767 (1996)

    PubMed  PubMed Central  Google Scholar 

  10. B. Pirzadeh, S. Dea, J. Gen. Virol. 79, 989 (1998)

    Article  CAS  Google Scholar 

  11. R.G. Bastos, O.A. Dellagostin, R.G. Barletta, A.R. Doster, O.A. Nelson, F.A. Osorio, Vaccine 21, 21 (2002)

    Article  CAS  Google Scholar 

  12. R.G. Bastos, O.A. Dellagostin, R.G. Barletta, A.R. Doster, O.A. Nelson, F. Zuckermann, F.A. Osorio, Vaccine 22, 467 (2004)

    Article  CAS  Google Scholar 

  13. A. Kheyar, A. Jabrane, C. Zhu, P. Cleroux, B. Massie, S. Dea, C.A. Gagnon, Vaccine 23, 4016 (2005)

    Article  CAS  Google Scholar 

  14. H. Qiu, Z. Tian, G. Tong, Y.J. Zhou, J.Q. Ni, Y.Z. Luo, X.H. Cai, Vet. Immunol. Immunopathol. 106, 309 (2005)

    Article  CAS  Google Scholar 

  15. Y. Jiang, S. Xiao, L. Fang, X. Yu, Y. Song, C. Niu, H. Chen, Vaccine 24, 2869 (2006)

    Article  CAS  Google Scholar 

  16. E.H.J. Wissink, M.V. Kroese, H.A.R. van Wijk, F.A.M. Rijsewijk, J.J.M. Meulenberg, P.J.M. Rottier, J. Virol. 79, 12495 (2005)

    Article  CAS  Google Scholar 

  17. P. Gonin, H. Mardassi, C.A. Gagnon, B. Massie, S. Dea, Arch. Virol. 143, 1927 (1998)

    Article  CAS  Google Scholar 

  18. H. Mardassi, P. Gonin, C.A. Gagnon, B. Massie, S. Dea, J. Virol. 72, 6298 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  19. J.J.M. Meulenberg, B.A. Petersen-Den, E.P. de Kluyvier, R.J. Moormann, W.M. Schaaper, G. Wensvoort, Virology 206, 155 (1995)

    Article  CAS  Google Scholar 

  20. Y. Zhou, T. An, J. Liu, H. Qiu, Y. Wang, G. Tong, Virus Res. 118, 98 (2005)

    Article  Google Scholar 

  21. J.B. Katz, A.L. Shafer, K.A. Eernisse, J.G. Landgraf, E.A. Nielson, Vet. Microbiol. 44, 65 (1995)

    Article  CAS  Google Scholar 

  22. J. Plana-Duran, I. Climent, J. Sarraseca, A. Urniza, E. Corteas, C. Vela, I. Casal, Virus Genes 14, 19 (1997)

    Article  CAS  Google Scholar 

  23. W. Jiang, P. Jiang, Y. Li, J. Tang, X. Wang, S. Ma, Vet. Immunol. Immunopathol. 113, 168 (2006)

    Article  Google Scholar 

  24. C.B. Bruce, A. Akrigg, S.A. Sharpe, T. Hanke, G.W.G. Wilkinson, M.P. Cranage, J. Gen. Virol. 80, 2621 (1999)

    Article  CAS  Google Scholar 

  25. E.M. Bautista, P. Suarez, T.W. Molitor, Arch. Virol. 144, 117 (1999)

    Article  CAS  Google Scholar 

  26. G. Rompato, E. Ling, Z. Chen, H. van Kruiningen, A.E. Garmendia, Vet. Immunol. Immunopathol. 109, 151 (2005)

    Article  Google Scholar 

  27. D. Ivanyi, J. Natl. Cancer Inst. 79, 149 (1987)

    CAS  PubMed  Google Scholar 

  28. K.M. Lager, W.L. Mengeling, in Proceedings of the Annual Meeting of the American Association of Swine Practitioners (1997), pp. 443–446

  29. F.A. Osorio, F. Zuckermann, R. Wills, in Proceedings of the 25th Anniversary Allen D. Leman Swine Conference, College of Veterinary Medicine, University of Minnesota (1998), pp. 176–182

  30. W.L. Mengeling, K.M. Lager, A.C. Vorwald, K.J. Koehler, Vet. Microbiol. 93, 13 (2003)

    Article  CAS  Google Scholar 

  31. X.J. Meng, Vet. Microbiol. 74, 309 (2000)

    Article  CAS  Google Scholar 

  32. S.M. Cancel-Tirado, R.B. Evans, K.J. Yoon, Vet. Immunol. Immunopathol. 102, 249 (2004)

    Article  CAS  Google Scholar 

  33. D.A. Benfield, E. Nelson, J.E. Collins, L. Harris, S.M. Goyal, D. Robison, W.T. Christianson, R.B. Morrison, D. Gorcyca, D. Chladek, J. Vet. Diagn. Invest. 4, 127 (1992)

    Article  CAS  Google Scholar 

  34. M.F. Callan, N. Steven, P. Krausa, J.D. Wilson, P.A. Moss, G.M. Gillespie, J.I. Bell, A.B. Rickinson, A.J. McMichael, Nature Med. 2, 906 (1996)

    Article  CAS  Google Scholar 

  35. A.E. Lukacher, V.L. Braciale, T.J. Braciale, J. Exp. Med. 160, 814 (1984)

    Article  CAS  Google Scholar 

  36. S.R. Ridell, P. Reusser, P.D. Greenberg, Rev. Infect. Dis. 13, S966 (1991)

    Article  Google Scholar 

  37. J.A. Byrne, M.B. Oldstone, J. Immunol. 136, 698 (1986)

    CAS  PubMed  Google Scholar 

  38. J. Castillo-Olivares, J.P. Tearle, F. Montesso, D. Westcott, J.H. Kydd, N.J. Davis-Poynter, D. Hannant, J. Gen. Virol. 84, 2745 (2003)

    Article  CAS  Google Scholar 

  39. P. Borrow, H. Lewicki, B.H. Hahn, G.M. Shaw, M.B. Oldstone, J. Virol. 68, 6103 (1994)

    CAS  PubMed  PubMed Central  Google Scholar 

  40. T.C. Greenough, D.B. Brettler, M. Somasundaran, D.L. Panicali, J.L. Sullivan, J. Infect. Dis. 176, 118 (1997)

    Article  CAS  Google Scholar 

  41. P.G. Stevenson, S. Hawke, C.R. Bangham, Virology 232, 158 (1997)

    Article  CAS  Google Scholar 

  42. J.W. Shiver, M.E. Davies, Y. Yasutmoni, H.C. Perry, D.C. Freed, N.L. Letvin, M.A. Liu, Vaccine 15, 884 (1997)

    Article  CAS  Google Scholar 

  43. E.M. Bautista, T.W. Molitor, Viral Immunol. 10, 83 (1997)

    Article  CAS  Google Scholar 

  44. L.L. Fuertes, N. Domenech, B. Alvarez, A. Ezquerra, J. Dominguez, J.M. Castro, F. Alonso, Virus Res. 64, 33 (1999)

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by Program for New Century Excellent Talents in University (NCET-04-0502), and grants from the National Natural Science Foundation (30270990, 30471288), Jiangsu Province Natural Science Foundation (BK2005093), and partly the Key Project of Chinese Ministry of Education (104101).

Author information

Authors and Affiliations

  1. Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, 210095, China

    Wenming Jiang, Ping Jiang, Yufeng Li, Xianwei Wang & Yijun Du

Authors
  1. Wenming Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ping Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yufeng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xianwei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yijun Du
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ping Jiang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, W., Jiang, P., Li, Y. et al. Analysis of immunogenicity of minor envelope protein GP3 of porcine reproductive and respiratory syndrome virus in mice. Virus Genes 35, 695–704 (2007). https://doi.org/10.1007/s11262-007-0143-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-007-0143-7

Keywords

Search

Navigation