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Structural polypeptides of the American (VR-2332) strain of porcine reproductive and respiratory syndrome virus

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Summary

The structural polypeptides of the isolate VR-2332 of porcine reproductive and respiratory syndrome virus were analyzed in sucrose gradient-purified virions. The virus had an average density of 1.15 g/cm3 and contained, by SDS-PAGE, three major polypeptides with apparent molecular weights of 15, 19 and 26–30 kDa, which were designated as nucleocapsid (N), matrix (M) and envelope (E), respectively. The predominant structural protein was N. N-glycosidase F digestion only affected E whereas O-glycosidase or endoglycosidase H digestion had no effect, suggesting that the viral glycoproteins contain only complex N-linked carbohydrates.

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Authors and Affiliations

  1. Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A.

    E. M. Bautista, C. S. Choi & T. W. Molitor

  2. Department of Virology, Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands

    J. J. M. Meulenberg

Authors
  1. E. M. Bautista
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  2. J. J. M. Meulenberg
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  3. C. S. Choi
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  4. T. W. Molitor
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Bautista, E.M., Meulenberg, J.J.M., Choi, C.S. et al. Structural polypeptides of the American (VR-2332) strain of porcine reproductive and respiratory syndrome virus. Archives of Virology 141, 1357–1365 (1996). https://doi.org/10.1007/BF01718837

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  • DOI: https://doi.org/10.1007/BF01718837

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