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Comparative analysis of the prion protein gene sequences in African lion

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Abstract

The prion protein gene of African lion (Panthera Leo) was first cloned and polymorphisms screened. The results suggest that the prion protein gene of eight African lions is highly homogenous. The amino acid sequences of the prion protein (PrP) of all samples tested were identical. Four single nucleotide polymorphisms (C42T, C81A, C420T, T600C) in the prion protein gene (Prnp) of African lion were found, but no amino acid substitutions. Sequence analysis showed that the higher homology is observed to felis catus AF003087 (96.7%) and to sheep number M31313.1 (96.2%) Genbank accessed. With respect to all the mammalian prion protein sequences compared, the African lion prion protein sequence has three amino acid substitutions. The homology might in turn affect the potential intermolecular interactions critical for cross species transmission of prion disease

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Acknowledgments

We thank Jia Junying, College of Veterinary Medicine, China Agricultural University, for the excellent technical support and dedication; Li Ru, Daliasn forest safri park, for the donation of African lion blood. This work was supported by the Natural Science Foundation of China (Project No. 30400325).

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Authors and Affiliations

  1. National Animal Transmissible Spongiform Encephalopathies Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China

    Chang-De Wu & De-Ming Zhao

  2. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Liaoning, 110161, China

    Chang-De Wu

  3. Faculty of Veterinary Medicine, University College Dublin, Dublin 4, Dublin, Ireland

    Wan-Yong Pang

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  1. Chang-De Wu
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  2. Wan-Yong Pang
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  3. De-Ming Zhao
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Correspondence to De-Ming Zhao.

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Wu, CD., Pang, WY. & Zhao, DM. Comparative analysis of the prion protein gene sequences in African lion. Virus Genes 33, 213–214 (2006). https://doi.org/10.1007/s11262-005-0058-0

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  • DOI: https://doi.org/10.1007/s11262-005-0058-0

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