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Tat-MyoD fused proteins, together with C2c12 conditioned medium, are able to induce equine adult mesenchimal stem cells towards the myogenic fate

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Abstract

The Tat protein is able to translocate through the plasma membrane and when it is fused with other peptides may acts as a protein transduction system. This ability appears particularly interesting to induce tissue-specific differentiation when the Tat protein is associated to transcription factors. In the present work, the potential of the complex Tat-MyoD in inducing equine peripheral blood mesenchymal stem cells (PB-MSCs) towards the myogenic fate, was evaluated. Results showed that the internalization process of Tat-MyoD happens only in serum free conditions and that the nuclear localization of the fused complex is observed after 15 hours of incubation. However, the supplement of Tat-MyoD only was not sufficient to induce myogenesis and, therefore, in order to achieve the myogenic differentiation of PB-MSCs, conditioned medium from C2C12 cells was added without direct contact. Real Time PCR and immunofluorescence methods evaluated the establishment of a myogenic program. Our results suggest that TAT- transduction of Tat-MyoD, when supported by conditioned medium, represents a useful methodology to induce myogenic differentiation.

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Acknowledgments

We thank Prof. Anthea Rowlerson (King’s College London, UK) for manuscript language revision. This work was supported by a grant from the University of Padova, Italy (PRAT 2013, code number CPDA138242).

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Authors and Affiliations

  1. Department of Comparative Biomedicine and Food Science, University of Padova, Padova, Italy

    Marco Patruno, Chiara Gomiero, Roberta Sacchetto & Tiziana Martinello

  2. Department of Biomedical Sciences, University of Padova, Padova, Italy

    Alessandro Negro

  3. VTH - Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel

    Ohad Topel & Alessandro Negro

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  1. Marco Patruno
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  2. Chiara Gomiero
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  3. Roberta Sacchetto
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Correspondence to Marco Patruno or Tiziana Martinello.

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Patruno, M., Gomiero, C., Sacchetto, R. et al. Tat-MyoD fused proteins, together with C2c12 conditioned medium, are able to induce equine adult mesenchimal stem cells towards the myogenic fate. Vet Res Commun 41, 211–217 (2017). https://doi.org/10.1007/s11259-017-9692-y

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