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Vagal afferent responses to cystometry in rat models of urinary bladder irritation: c-fos immunohistochemistry study

  • Urology - Original Paper
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Abstract

Purpose

Although recent literature provides increasing evidence concerning urinary bladder innervation by vagal afferents, the functional aspects and the conditions at which these afferents are recruited are still unclear.

Methods

In the present study, the neuronal responses of nodose ganglion following cystometry, under different models of rat’s urinary bladder irritation, cyclophosphamide (CYP), cyclophosphamide with cervical vagotomy (Vx), chronic HCl, and acute HCl, were investigated using c-fos immunohistochemistry.

Results

The c-fos expression in the nodose ganglion, following cystometry, was increased significantly in the CYP and chronic-HCl groups compared to the intact, Vx, and acute-HCl groups. In addition, the acute-HCl group showed a significant increase compared to intact animals. Following cervical vagotomy, the expression in the Vx group decreased significantly compared to the CYP group, but was significantly higher than that in the intact group.

Conclusion

The results of this study demonstrate the innervation of the vagus afferents to the urinary bladder. This innervation is activated under urinary bladder irritation conditions, which may indicate a possible role of the vagus nerve during urinary bladder pathology.

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Data availability

All data experimental data are available upon request.

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Acknowledgements

The research reported in this publication was funded by the Deanship of Scientific Research and Innovation at Al-Balqa Applied University in Jordan under Award Number 58/2018/2019.

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Authors and Affiliations

  1. Department of Basic Medical Sciences, Faculty of Medicine, Al-Balqa Applied University, Al-Salt, 19117, Jordan

    Ezidin G. Kaddumi & Manal Udwan

  2. Department of Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University, Al-Salt, Jordan

    AbdulFattah S. Fararjeh

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  1. Ezidin G. Kaddumi
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  2. AbdulFattah S. Fararjeh
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  3. Manal Udwan
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Correspondence to Ezidin G. Kaddumi.

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Kaddumi, E.G., Fararjeh, A.S. & Udwan, M. Vagal afferent responses to cystometry in rat models of urinary bladder irritation: c-fos immunohistochemistry study. Int Urol Nephrol 56, 69–75 (2024). https://doi.org/10.1007/s11255-023-03791-y

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  • DOI: https://doi.org/10.1007/s11255-023-03791-y

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