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Decellularized dermal strip as a suburethral sling in a rat model of stress urinary incontinence

  • Urology - Original Paper
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Abstract

Introduction

To report the feasibility, efficacy and safety of decellularized dermal strip as a sling material in the pudendal denervated stress urinary incontinence (SUI) in an animal model.

Methods

Thirty female Sprague–Dawley rats were randomly allocated into three equal groups (n = 10). In group 1, rats underwent bilateral pudendal nerve transection (BPNT) with no vaginal sling placement. Group 2 underwent BPNT with homologous decellularized dermal strip placement as vaginal sling 12 weeks later. Group 3 was considered as controls to evaluate baseline leak point pressure (LPP). Leak point pressure was measured 18 weeks after BPNT in group 1 and 6 weeks after sling implantation in group 2. Sling and sphincter samples were prepared for histological and immunohistochemistry (IHC) staining.

Results

H&E staining of external urethral sphincter showed decrease in striated muscle layer in group 1 (BPNT) compared to control confirmed by desmin staining (45 % decrease in desmin staining). Sling implantation caused significant increase in mean LPP from 11.6 ± 2.8 cm H2O in group 1 (BPNT) to 27.2 ± 5.4 cm H2O in group 2 (BPNT/sling). There was no significant difference between LPP after the sling placement in group 2 and LPP in group 3 (27.2 ± 5.4 cm H2O vs. 27.6 ± 5.9, P = 0.832). IHC staining was positive for α-SMA and CD34.

Conclusions

Based on present results, this dermal strip could be considered as a potential sling material for treatment of SUI in this animal model. This study provides the basis for further investigation of the efficacy of biological decellularized scaffold as suburethral sling material.

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Conflict of interest

Abdol-Mohammad Kajbafzadeh, Sarah Mozafarpour, Seyedeh Sanam Ladi Seyedian, Reza Khorramirouz and Haleh Nasser Hojjati declare that they have no conflict of interest.

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Authors and Affiliations

  1. Section of Tissue Engineering and Stem Cells Therapy, Pediatric Urology Research Center, Children’s Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Qarib’s Street, Keshavarz Boulevard, 1419433151, Tehran, Iran (IRI)

    Abdol-Mohammad Kajbafzadeh, Sarah Mozafarpour, Seyedeh Sanam Ladi Seyedian & Reza Khorramirouz

  2. Department of Urology, Ziaeian Hospital, Tehran University of Medical Sciences, Tehran, Iran (IRI)

    Haleh Nasser Hojjati

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  1. Abdol-Mohammad Kajbafzadeh
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  2. Sarah Mozafarpour
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  5. Haleh Nasser Hojjati
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Correspondence to Abdol-Mohammad Kajbafzadeh.

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Kajbafzadeh, AM., Mozafarpour, S., Ladi Seyedian, S. et al. Decellularized dermal strip as a suburethral sling in a rat model of stress urinary incontinence. Int Urol Nephrol 47, 1303–1310 (2015). https://doi.org/10.1007/s11255-015-1025-x

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  • DOI: https://doi.org/10.1007/s11255-015-1025-x

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