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Application of amniotic membrane as xenograft for urethroplasty in rabbit

  • Urology - Original Paper
  • Published:
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Abstract

Objectives

Uroepithelium repair has always been the subject of discussion in urology surgeries. Using human amniotic membrane allograft has already proved to be useful in other fields. In this study, we use amniotic membrane to repair uroepithelium injuries in rabbits.

Methods

Twenty healthy rabbits underwent surgery to induce a 10-mm incision, 10 mm from the meatus on ventral part of the urethra. Then a 5 × 10 mm patch of amniotic membrane was sutured to the incised urethra. All of the rabbits were catheterized for a week and then the patches were removed. The rabbits were studied for a month for any signs of infection and fistula formation.

Results

All samples revealed complete re-epithelialization of reconstructed urethra by transitional epithelium. There was one case of infection and following fistula (5%). There were two cases of urethral strictures (10%).

Conclusion

The result from this study suggests that amniotic membrane is an inexpensive, easy, and biodegradable graft with very little antigen effect which seems to be the ideal solution for urethroplasty.

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Acknowledgement

This study was funded by The Shiraz Nephro-Urology Research Center of Shiraz University of Medical Sciences.

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Authors and Affiliations

  1. Department of Surgery, Division of Urology, Shahid Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

    Saeed Shakeri, Abdolreza Haghpanah & Abdolaziz Khezri

  2. Department of Obstetrics and Gynecology, Shahid Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

    Maryam Yazdani

  3. Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran

    Ahmad Monabbati

  4. Department of Health System Research, Shiraz University of Medical Sciences, Shiraz, Iran

    Sezaneh Haghpanah

  5. Department of Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Leila Malekmakan & Soroush Ayrempour

Authors
  1. Saeed Shakeri
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  2. Abdolreza Haghpanah
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  3. Abdolaziz Khezri
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  4. Maryam Yazdani
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Correspondence to Sezaneh Haghpanah.

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Shakeri, S., Haghpanah, A., Khezri, A. et al. Application of amniotic membrane as xenograft for urethroplasty in rabbit. Int Urol Nephrol 41, 895–901 (2009). https://doi.org/10.1007/s11255-009-9532-2

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  • DOI: https://doi.org/10.1007/s11255-009-9532-2

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