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Safety and efficacy of human amniotic membrane in primary pterygium surgery

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Abstract

Grafts made from human amniotic membrane are used to prevent recurrence of pterygium after excision. The success of the procedure can be affected by the quality of preparation and preservation of the grafts. We prospectively evaluated the safety and efficacy of cryopreserved amniotic membrane prepared at the research tissue bank of the Biotechnology Research Center in Tripoli, Libya, and used as adjunct therapy in primary pterygium excision. Twenty-six patients (15 males and 11 females) aged 21–78 years and indicated for primary pterygium excision were transplanted at the Tripoli Eye Hospital with the amniotic membrane grafts. Sixteen patients (62 %) were available for all three follow-up visits scheduled at 1, 3 and 6 months post-surgery. By the third visit, two patients (12.5 %) developed granuloma and three (18.8 %) had pterygium recurrence. The grafts were used after cryopreservation for ≤180 days or >180 days, but statistical analysis showed that the complications were not associated with the length of storage. Moreover, the high rate of complications in this study was not caused by use of cryopreserved AM. In conclusion, locally produced cryopreserved AM is safe as an adjunct therapy for treatment of primary pterygium excision.

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Acknowledgments

This work was funded by the Libyan Authority for Research Science and Technology, Tripoli Libya. The authors thank the Biotechnology Research Centre for providing the equipment and materials used in this study. Thanks to Dr. Amin Bredan for his valuable editing of the manuscript, and thanks are due to Dr. Abdulmunem Abulayha, Dr. Abdul Munam Fellah and Mohamed Mansur for their assistance.

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Authors and Affiliations

  1. Tissue Banking Research Group, Biotechnology Research Centre (BTRC), Tweisha, Libyan Authority for Research, Sciences and Technology, P. O. Box 30313, Tripoli, Libya

    Nagi Marsit, Adel Mabrouk & Sedigh Abdalla

  2. Tripoli Eye Hospital, Tripoli, Libya

    Nagmeden Gafud, Inass Kafou, Aml Alatiweel & Lamia Sheghewi

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  1. Nagi Marsit
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  2. Nagmeden Gafud
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  3. Inass Kafou
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  4. Adel Mabrouk
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  5. Aml Alatiweel
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  6. Sedigh Abdalla
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  7. Lamia Sheghewi
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Correspondence to Nagi Marsit.

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Marsit, N., Gafud, N., Kafou, I. et al. Safety and efficacy of human amniotic membrane in primary pterygium surgery. Cell Tissue Bank 17, 407–412 (2016). https://doi.org/10.1007/s10561-016-9554-9

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  • DOI: https://doi.org/10.1007/s10561-016-9554-9

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