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Effects of Carica papaya peel meal-based diets on rabbit bucks’ spermiogram and reaction time

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Abstract

The study aimed to evaluate the effects of Carica papaya peel meal-based diet on spermiogram and reaction time in rabbit bucks. Ripe pawpaw fruits were harvested during the dry season. The peels were carefully removed from the pulp and sun-dried for a week. Afterward, they were ground and included in the test diets as pawpaw peel meal (PPM) at inclusion rates of 0%, 15%, and 30%. Rabbit bucks (n = 15) were randomly separated into three groups of five bucks and labeled as groups A, B, and C. Group A, the control group (0%), was fed the basal protein diet (BD), group B (PPM 15) was given a PPM-based diet (15%), while C (PPM 30) was given diet composed of PPM (30%). Semen samples were collected and evaluated fortnightly for 14 weeks. The reaction time and mean ejaculate volume were lower (P < 0.05) in the treatment groups than in the control. Sperm motility and concentration decreased significantly (P < 0.05) across the groups from week 4 to the end of the experiment. Bucks fed PPM 15%, and PPM 30% had significantly (P < 0.05) higher percentages of dead sperm cells and total spermatozoa abnormalities. The control had (86%) normal spermatozoa morphology while those of PPM 15% and PPM 30% were (61%) and (52%), respectively. PPM 30% had the highest abnormal spermatozoa (47%) compared to PPM 15% (38%) and control (13%). The findings indicate that pawpaw peels up to 15% and 30% in the diet have a negative effect on spermiogram.

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Acknowledgements

The authors appreciate the NAPRI employees, especially those from the Artificial Insemination Unit who helped with the laboratory work and the entire team of the NAPRI Rabbit Unit of the Swine and Rabbit Research Program.

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Authors and Affiliations

  1. Department of Theriogenology and Production, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

    Ibrahim Shettima, Peter Ibrahim Rekwot & Agnes Ifeoma Nwannenna

  2. Department of Animal Science, Faculty of Agriculture, Federal University of Kashere, Gombe, Gombe State, Nigeria

    Ibrahim Shettima

  3. Department of Veterinary Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

    Joseph Olusegun Ayo

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  1. Ibrahim Shettima
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Contributions

Ibrahim Shettima: conceptualization, resources, data curation, formal analysis, and writing—original draft. Peter Ibrahim Rekwot: conceptualization, methodology, supervision, investigation, and writing—original draft. Joseph Olusegun Ayo: conceptualization, supervision, writing, review, editing, and investigation. Agnes Ifeoma Nwannenna: supervision and investigation.

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Correspondence to Ibrahim Shettima.

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The Animal Use and Care Committee of Ahmadu Bello University approved the experiment and assigned the ABUCAUC/2022/054 reference number.

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Shettima, I., Rekwot, P.I., Ayo, J.O. et al. Effects of Carica papaya peel meal-based diets on rabbit bucks’ spermiogram and reaction time. Trop Anim Health Prod 56, 84 (2024). https://doi.org/10.1007/s11250-024-03911-5

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