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Dietary supplementation with Piper sarmentosum extract on gut health of chickens infected with Eimeria tenella

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Abstract

To explore the potential alternative of anti-coccidials, we investigated the therapeutic efficacy of dietary Piper sarmentosum extract (PSE) on induced coccidia infection in chickens. A total of 96-day-old chickens were randomly distributed to 1 of 3 treatment groups, including (1) control negative untreated uninfected (CN), (2) control positive untreated infected (CP), and (3) Piper sarmentosum (P. sarmentosum) extract-treated infected group (PSE). Our results demonstrated that E. tenella challenged untreated group showed a reduction (P < 0.05) in post-infection (PI) body weight compared to control negative group. However, supplementation of P. sarmentosum extract had no significant effects on body weight and cecal lesions compared with control positive group. Infected chickens fed PSE diet decreased (P < 0.05) the bloody diarrhea scores and oocyst shedding (during the day 5 to 8 post-infection) than that of CP chickens. E. tenella–challenged chickens upregulated (P < 0.05) the mRNA expression of IL-8 and Bcl-2 compared to PSE chickens, while IFN-γ compared to CN chickens. On the other hand, treatment of P. sarmentosum extract tended to increase (P < 0.05) the transcription patterns of IL-4, IL-10, CLDN 1, SOD 1, and Bax with the comparison of control positive group; however, there were no significant effects on IL-8, ZO 1, and CAT expression between the PSE and CP groups. Collectively, these findings elaborated that dietary P. sarmentosum extract exhibit potential anti-coccidial effects in controlling the coccidia infection in chickens.

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Acknowledgements

The authors are grateful to the technical staff of the Department of Clinical Veterinary Medicine, Collage of Animal Sciences and Technology, Guangxi University, for their valuable support in conducting the current experiment.

Funding

This work was supported by Science and Technology Major Project of Guangxi, China (AA17204057); the Key Research and Development Plan of Guangxi, China (AB19245037); Natural National Science Foundation of China (317607446); and  the Major R&D Project of Nanning Xingning District(2020A14);

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Authors and Affiliations

  1. Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning, China

    Fareed Uddin Memon, Yunqiao Yang, Ahmed M. Soliman, Feifei Lv, Geyin Zhang, Yuhan Wang & Hongbin Si

  2. Biotechnology Department, Animal Health Research Institute, Agricultural Research Center, Dokki, Giza, Egypt

    Ahmed M. Soliman

  3. Department of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agricultural University, Tando Jam, Pakistan

    Nasir Rajput

  4. Collage of Electrical Engineering, Guangxi University, Nanning, China

    Mirza Baber Baig

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  1. Fareed Uddin Memon
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  2. Yunqiao Yang
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  3. Ahmed M. Soliman
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  4. Feifei Lv
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Contributions

Fareed Uddin Memon and Yunqiao Yang conducted the all experiments. Hongbin Si was the principal investigator for all experimental conditions and reviewed the final draft of manuscript. Geyin Zhang and Yuhan Wang contributed by performing the laboratory experiments. Mirza Baber Baig contributed in data analysis. Ahmed M. Soliman and Nasir Rajput reviewed and revised the final draft of manuscript.

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Correspondence to Hongbin Si.

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The experimental protocols for the chickens were reviewed and approved by the Animal Care and Use Committee of Guangxi University, Nanning, China.

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Memon, F.U., Yang, Y., Soliman, A.M. et al. Dietary supplementation with Piper sarmentosum extract on gut health of chickens infected with Eimeria tenella. Trop Anim Health Prod 53, 497 (2021). https://doi.org/10.1007/s11250-021-02934-6

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