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First molecular epidemiological study of Mycobacterium avium subsp. paratuberculosis in cattle and buffalo from different regions of Brazil

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Abstract

Paratuberculosis is an incurable disease in ruminants with great worldwide economic impact, caused by Mycobacterium avium subsp. paratuberculosis (MAP). The objective of this study was to carry out a study of the molecular epidemiology of the MAP using the restriction enzyme analysis (REA) technique of IS1311 MAP region in biological samples of feces, intestinal tissue, and mesenteric lymph nodes of cattle and buffaloes from six Brazilian states. In total, 109 samples of feces and tissues of cattle and buffaloes were collected from animal paratuberculosis suspected. Twenty-five samples were positive in the detection of the DNA of the IS900 region of MAP and it was possible to type 18 strains in the analysis of the region IS1311, being 100% of them identified as belonging to subtype Bison MAP strain. This is the first epidemiological molecular study of MAP in Brazil. The results indicate that paratuberculosis is widespread in cattle and in buffaloes in several regions of Brazil, and the subtype Bison MAP strain was the only one identified in the samples analyzed in this study, demonstrating the similarity between the strains from different states tested. These results provide the necessary support for the implementation of paratuberculosis control strategies in cattle and buffaloes in Brazil.

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Authors and Affiliations

  1. Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, PE, Brazil

    Pedro Paulo Feitosa de Albuquerque, Renata Pimentel Bandeira de Melo & Rinaldo Aparecido Mota

  2. Department of Epidemiology and Public Health, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Marilene de Farias Brito

  3. Faculty of Agrarian Sciences of Andradina, Educational Foundation of Andradina, Andradina, SP, Brazil

    Fernanda Bovino

  4. Federal University of Uberlândia, Campus Umuarama, Uberlandia, MG, Brazil

    Mariana Assunção de Souza & Anna Monteiro Correia Lima

  5. Clinical Department, State University of Maranhão, São Luiz, MA, Brazil

    Emerson Antônio Araújo de Oliveira & Helder de Moraes Pereira

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  1. Pedro Paulo Feitosa de Albuquerque
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  2. Renata Pimentel Bandeira de Melo
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  3. Marilene de Farias Brito
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  4. Fernanda Bovino
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  5. Mariana Assunção de Souza
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  6. Anna Monteiro Correia Lima
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  7. Emerson Antônio Araújo de Oliveira
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  8. Helder de Moraes Pereira
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  9. Rinaldo Aparecido Mota
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Correspondence to Pedro Paulo Feitosa de Albuquerque.

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This study was approved by the Ethics Committee for the Use of Animals of the Federal Rural University of Pernambuco under license no. 123/2015.

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de Albuquerque, P.P.F., de Melo, R.P.B., de Farias Brito, M. et al. First molecular epidemiological study of Mycobacterium avium subsp. paratuberculosis in cattle and buffalo from different regions of Brazil. Trop Anim Health Prod 50, 1929–1935 (2018). https://doi.org/10.1007/s11250-018-1650-3

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