Abstract
Purpose
Coccidiosis is an important parasitic disease in poultry industry. Owing to the development of drug resistance against Eimeria and concerns about drug residues, attentions toward the alternative compounds including herbal medicines have been increased. This study aimed to examine the prophylactic and therapeutic effects of a new formulation derived from Artemisia sieberi extract on avian coccidiosis.
Methods
The extract was obtained from A. sieberi using petroleum ether, and then, it was formulated into a wet granule. Three hundred and sixty 1-day-old broilers were divided into six groups, each with three replicates (n = 20); the first group was chosen as the noninfected control group and the remainders were challenged by oral administration of 250,000 oocysts of Eimeria tenella per chick on day 21. Group 2 (as nontreated control) received no treatment, but group 3 (as prophylactic group) received 0.5 mg/kg artemisinin 10 days before challenging with E. tenella. However, groups 4, 5, and 6 received 1 mg/kg artemisinin on the next day and at 24 and 48 h after the challenge, respectively, for 5 days. Mortality rate, bloody diarrhea score, lesion score, and oocysts per gram (OPG) of feces were used to determine the anticoccidial effects of the formulation.
Results
The granule significantly (p < 0.05) reduced mortality, diarrhea, and lesion score in the treated groups. The OPG output was also significantly (p < 0.0001) reduced in the groups that received formulation. However, there was no significant difference between the prophylactic and therapeutic groups.
Conclusion
The results suggested that this new formulation is a promising herbal medicine that can be used as a prophylactic or therapeutic product to control avian coccidiosis.
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References
Allen, P.C., Danforth, H.D., Levander, O.A., 1996. Diets high in n-3 fatty acids reduce cecal lesion scores in chickens infected with Eimeria tenella. Poultry Science, 75, 179.
Allen, P.C., Danforth, H., Levander, O.A., 1997a. The effects of high n-3 fatty acids on the ultra-structural development of Eimeria tenella. Parasitology Research, 83, 440–444.
Allen, P.C., Lydon, J., Danforth, H.D., 1997b. Effects of components of Artemisia annua on coccidia infections in chickens. Poultry Science, 76, 1156.
Allen, P.C., Danforth, H.D., Augustine, P.C., 1998. Dietary modulation of avian coccidiosis. International Journal of Parasitology, 28, 1131–1140.
Allen, P.C., Fetter, R.H., 2002. Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clinical Microbiology Review, 15, 58–65.
Almeida, G, F., Horsted, K., Thamsborg, S, M., Kyvsgaard, N, C., Ferreira, J, F, S., Hermansen, J, E., 2012. Use of Artemisia annua as a natural coccidiostat in free range broiler and its effects on infection dynamics and performance. Veterinary Parasitology, 186, 178–187.
Arab, H.A., Kaboutari Katadj, J, Rahbari, S., Nibian, S., SoltanMohammadi, A.R., Pirali Kheirabadi KH., 2012. Comparison between anticoccidial effect of granulated Artemisia sieberi extract and pure artemisinin in affected broiler. Journal of Veterinary Research, 67(2), 119–125.
Arab, H.A., Rahbari, S., Rassouli, A., Moslemi, M.H., Khosravirad, F., 2006. Determination of artemisinin in Artemisia sieberi and anticoccidial effects of the plant extract in broiler chickens. Tropical Animal Health and Production, 38, 497–503.
Brisibe, E.A., Umoren, U, E., Owai, P.U., Brisibe, F., 2008. Dietary inclusion of dried Artemisia annua leaves for management of coccidiosis and growth enhancement in chickens. African Journal of Biotechnology, 7, 4083–4092.
Conway, D.P., McKenzie, E., 1991. Poultry coccidiosis—Diagnosis and testing procedure (Pfizer)
Dalloul, R.A., Lillehoj, H.S., 2006. Poultry coccidiosis: recent advances in control measures and vaccine development. Expert Review of Vaccines, 5, 143–163.
del Cacho, E., Gallego, M., Francesch, M., Quilez, J., Sanchez-Acedo, C., 2010. Effects of artemisinin on oocyst wall formation and sporulation during Eimeria tenella infection. International Parasitology, 50, 506–511.
Eckstein, L, U., Webb. R.J., Van Geothem. I.D.A., East, J.M., Lee, A.G., Kimura, M., et al., 2003. Artemisinin target SERCA of Plasmodium falciparum. Nature, 424, 957–961.
Holdsworth, P.A., Conway, D.P., McKenzie, M.E., Dayton, A.D., Chapman, R.B., Mathis, G.F., Skinner, J.T., Mundt, H.C., Williams, R.B., 2004. Proceeding of the World association for the advancement of veterinary parasitology (WAAVP) guidelines for evaluating the efficacy of anticoccidial drugs in chickens and turkeys. Veterinary Parasitology, 121, 189–212.
Hovnanian, A., 2007. SERCA pumps and human diseases. Subcellular Biochemistry, 45, 337–363.
Klayman, D.L., 1985. Qinghaosu (artemisinin): an antimalarial drug from China. Science, 228, 1049.
Krungkrai, S.R., Yuthavong, Y., 1987. The antimalarial action on Plasmodium falciparum of qinghaosu and artesunate in combination with agents which modulate oxidant stress. Transactions of the Royal Society of Tropical Medicine and Hygiene. 81, 710–714.
Levander, O.A., Ager, A.L., Morris, V.C., May, R.G., 1989. Qinghaosu, dietary vitamin E, selenium, and cod-liver oil: effect on the susceptibility of mice to the malarial parasite Plasmodium yoelii. The American Journal of Clinical Nutrition, 50, 346.
Liersch, R., Soicke, H., Stehr, C., Tullner, H, U., 1986. Formulation of artemisinin in Artemisia annua during one vegetation period. Planta Medica, 52, 387–390.
Meshnick, S.R., Tsang, T.W., Lin, F.B., Pan, H.Z., Chang, C.N., Kuypers, F., Chiu, D., Chiu, D., Lubin, B., Eaton, J.W., Brewer, G.J., 1989. Activated oxygen mediates the antimalarial activity of qinghaosu. Progress in Clinical Biochemistry Research, 313, 95–104.
Nagamune, K., Moreno, S.N., Chini, E.N., Silbey, L.D., 2008. Calcium regulation and signaling in apicomplexan parasites. In: Beruleigh, B.A., Soldati-Favreb, D., Editors. Molecular mechanisms of parasite invasion. Subcellular Biochemistry, 47, 70–82
Nagamune, K., Beatty, W.L., Sibley, D.L., 2007. Artemisinin induces calcium dependent protein secretion in the protozoan parasite Toxoplasma gondii. Eukaryotic Cell, 6, 2147–2156.
Niadoo, V., McGaw, L.J., Bishop, S.P.R., Duncan, N., Ellof, N.J., 2008. The value of plant with antioxidant activity in attenuating coccidiosis in broiler chickens. Veterinary Parasitology, 153, 214–219.
Oh, H.G., Youn, H.J., Noh, J.W., Jang, D.H., Kang,Y.B., 1995a. Anticoccidial effects of an extract of Artemisia annua on the Eimeria tenella. Korean Journal of Veterinary Research, 35, 115–121.
Oh, H.G., Youn, H.J., Noh, J.W., Jang, D.H., Kang, Y.B.,1995b. Anticoccidial effects of Artemisinin on the Eimeria tenella. Korean Journal of Veterinary Research, 35, 123–130.
Olliaro, P. L., Taylor, W. R. J.2004., Developing artemisinin based drug combinations for the treatment of drug resistant falciparum malaria: a review. Journal of Postgraduate Medicine, 50, 40–44.
Weinberg, E.D., Moon, J., 2009. Malaria and iron: history and review. Drug Metabolism Review, 41, 644–662.
Willcox, M., Falquet, J., Ferreira, J.F.S., Glibert, B.H.S.U., Magalhaes, E., de, P.M., Plaizer –Vercammen, J., Wright, C.W., 2007. Artemisia annua as a herbal tea for malaria. African Journal of Traditional Complementary and Alternative Medicine, 4, 121–123.
Woodrow, C.J., Haynes, R.K., Krishna, S., 2005. Artemisinins. Journal of Postgraduate Medicine, 81, 71–78
Ying-Zi, Y., Little, B., Meshnick, S.R., 1994. Alkylation of proteins by artemisinin: Effects of heme, pH, and drug structure. Biochemical Pharmacology, 48, 569–573.
Youn, H.J., Kang, Y.B., Jang, D.H., 1993. Effects of γ irradiation from pathogenecity of Eimeria tenella. Korean Journal of Veterinary Parasitology, 33, 649–655.
Youn, H.J., Noh, J.W., 2001. Screening of the anticoccidial effects of barbs extracts against Eimeria tenella. Veterinary Parasitology, 96, 257–263.
Zhang, Z., Zeng, M., 2005. Research advances of drug resistance in chickens' coccidian. Chinese Journal of Veterinary Parasitology, 13, 29–36.
Zhu D., 1987. Recent advances on the active components in Chinese medicines. Abstracts of Chinese Medicine, 1(2), 251–266.
Acknowledgments
We appreciate the National Science Foundation (INSF), Shahrekord University, and Faculty of Veterinary Medicine, University of Tehran for their support to conduct this study, and we also thank Mr. Abbas Gerami for his technical assistant.
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Kaboutari, J., Arab, H.A., Ebrahimi, K. et al. Prophylactic and therapeutic effects of a novel granulated formulation of Artemisia extract on broiler coccidiosis. Trop Anim Health Prod 46, 43–48 (2014). https://doi.org/10.1007/s11250-013-0444-x
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DOI: https://doi.org/10.1007/s11250-013-0444-x