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Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

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Abstract

Biofilm formation in Staphylococcus aureus is considered an important virulence factor in bovine mastitis. Intercellular adhesion gene A (icaA) is a significant genetic determinant that contributes in biofilm formation. The aim of the present study was to determine the presence of the icaA gene in S. aureus isolated from bovine mastitis from seven states of India. A total of 88 out of 150 Staphylococcus aureus strains were found to be positive for biofilm marker icaA gene by PCR. The icaA gene was confirmed by dot blot hybridization in 41 of 150 S. aureus strains tested. Results obtained with dot blot hybridization were comparable to those obtained with PCR. Partial sequences of the icaA gene of the two S. aureus isolates showed deletion of some bases in different positions that might reduce/stop transcription leading to no biofilm formation. PCR was found to be a rapid test but dot blot hybridization was more accurate than PCR for detection of icaA genes. This study showed that detection of biofilm marker the icaA gene in S. aureus would allow the detection of virulence factors present in mastitis and early application of corrective measures.

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Acknowledgments

The authors are thankful to the Director, National Environmental Engineering Research Institute, Nagpur, India and Dr. A. G. Bhandarkar, Professor and Head, Department of Veterinary Pathology, Nagpur Veterinary College, Nagpur, India for providing the facilities to conduct this research.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Animal Biotechnology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur, 440006, India

    Somanath Kundalik Chavhan, Dewanand Rajaram Kalorey & Anshuja Arunkumar Nagdive

  2. Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, India

    Hemant J. Purohit

  3. ICAR Research Complex for Goa, Old Goa, 403402, India

    Sukhadeo B. Barbuddhe

  4. Department of Veterinary Pathology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur, 440006, India

    Nitin Vasantrao Kurkure

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  1. Somanath Kundalik Chavhan
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  2. Dewanand Rajaram Kalorey
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  3. Anshuja Arunkumar Nagdive
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  4. Hemant J. Purohit
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  5. Sukhadeo B. Barbuddhe
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  6. Nitin Vasantrao Kurkure
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Correspondence to Nitin Vasantrao Kurkure.

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Chavhan, S.K., Kalorey, D.R., Nagdive, A.A. et al. Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk. Trop Anim Health Prod 44, 247–252 (2012). https://doi.org/10.1007/s11250-011-0009-9

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  • DOI: https://doi.org/10.1007/s11250-011-0009-9

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