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Detection of intercellular adhesion genes and biofilm production in Staphylococcus aureus isolated from bovine subclinical mastitis

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Abstract

Biofilm production by Staphylococcus aureus, an important virulence factor was investigated employing phenotypic and genotypic methods. A total of 102 S. aureus isolates from bovine subclinical mastitis cases were included in the study. Maximum number of biofilm producing strains were detected by Congo red agar (CRA) method (48.03%) followed by tube method (36.27%). Tissue culture plate method (TCP) without and with destaining identified 19.60 and 29.41% of S. aureus as biofilm producers, respectively. A polymerase chain reaction for detection of intercellular adhesion genes, icaA and icaD, responsible for biofilm formation was standardized. Of the 102 S. aureus isolates investigated, 36 (35.29%) strains revealed presence of both the genes. Considering polymerase chain reaction as a standard test, CRA and TCP without destaining were the most sensitive and specific, respectively. PCR technique standardized for detection of the icaA and icaD genes is reliable for identifying biofilm producing potential of S. aureus which may help in rapid detection of biofilm-producer Staphylococci. This would allow the early application of control measures.

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Fig. 1
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Abbreviations

CMT:

California Mastitis Test

CRA:

Congo red agar

MTCC:

Microbial Type Culture Collection

PCR:

Polymerase chain reaction

SCM:

Subclinical mastitis cases

TCP:

Tissue culture plate method

icaA:

Intercellular adhesion gene A

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Authors and Affiliations

  1. Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur, 440 006, India

    Nirmala B. Dhanawade, Dewanand R. Kalorey & Nitin V. Kurkure

  2. Madras Veterinary College, Tamilnadu University of Veterinary and Animal Sciences, Chennai, 600 006, India

    R. Srinivasan

  3. ICAR Research Complex for Goa, ELA, Old Goa, 403 402, India

    Sukhadeo B. Barbuddhe

Authors
  1. Nirmala B. Dhanawade
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  2. Dewanand R. Kalorey
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  3. R. Srinivasan
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  4. Sukhadeo B. Barbuddhe
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  5. Nitin V. Kurkure
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Corresponding author

Correspondence to Sukhadeo B. Barbuddhe.

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Dhanawade, N.B., Kalorey, D.R., Srinivasan, R. et al. Detection of intercellular adhesion genes and biofilm production in Staphylococcus aureus isolated from bovine subclinical mastitis. Vet Res Commun 34, 81–89 (2010). https://doi.org/10.1007/s11259-009-9326-0

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  • DOI: https://doi.org/10.1007/s11259-009-9326-0

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