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The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat

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Abstract

Although particle bombardment is the predominant method of foreign DNA direct transfer, whether transgene is integrated randomly into the genome has not been determined. In this study, we identified the distribution of transgene loci in 45 transgenic wheat (Triticum aestivum L.) lines containing co-transformed high molecular weight glutenin subunit genes and the selectable marker bar using fluorescence in situ hybridization. Transgene loci were shown to distribute unevenly throughout the genome and incorporate into different locations along individual chromosomes. There was only a slight tendency towards the localization of transgenes in distal chromosome regions. High proportions of transgenes in separate plasmids integrated at the same site and only 7 lines had 2 or 3 loci. Such loci may not segregate frequently in subsequent generations so it is difficult to remove selectable markers from transgenic lines after regeneration. We also found that three transgene lines were associated with rearranged chromosomes, suggesting a the close relationship between particle bombardment-mediated transgene integration and chromosomal rearrangements.

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Acknowledgments

This research was supported by the financial support from China Postdoctoral Science Foundation to Yonghua Han (20090460049 and 201003181) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China

    Yonghua Han

  2. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Yonghua Han & Daowen Wang

  3. Western Regional Research Center, USDA-ARS Crop Improvement and Genetics, Albany, CA, 94710, USA

    Ann Blechl

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  1. Yonghua Han
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  2. Ann Blechl
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  3. Daowen Wang
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Correspondence to Yonghua Han or Daowen Wang.

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Han, Y., Blechl, A. & Wang, D. The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat. Transgenic Res 24, 1055–1063 (2015). https://doi.org/10.1007/s11248-015-9906-4

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