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Biocatalytic Conversion of Lignocellulosic Water Hyacinth Biomass by Phanerochaete chrysosporium for Sustainable Ethanol Production

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Abstract

Water hyacinth (Eichhornia crassipes) as a aquatic weed has become a source of concern for value addition. This study aimed to determine the feasibility of the weedy biomass in sustainable bioethanol production using Phanerochaete chrysosporium and Saccharomyces cerevisiae. The results indicated that P. chrysosporium significantly utilized 70.9% of cellulose and 70% of hemicellulose from raw lignocellulose of water hyacinth with significant microbial enzyme production of 1.26 IU/ml. Moreover, the microbial treatment resulted in a significant amount of soluble protein (194.30 mg/g) and reducing sugar (34.20 g/l). XRD, SEM and FTIR analyses revealed that the crystalinity of cellulose was increased with the microbial treatment and hence, the yield of sugar also. Under submerged fermentation, Saccharomyces cerevisiae produced a maximum of 20.17 g/l of ethanol. The promising results of the present study explored the microbial treatment with P. chrysosporium and fermentation with S. cerevisiae as a successful and sustainable method for ethanol production from lignocellulosic weedy biomass.

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Acknowledgements

The authors extend their appreciation to Researchers Supporting Project Number (RSP2024R165), Kind Saud University, Riyadh, Saudi Arabia. The authors thankfully recognize the Department of Botany, Government Arts and Science College, Melur and the support of the management of MGR College in Hosur, Tamilnadu, India.

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Authors and Affiliations

  1. PG and Research Department of Botany, Alagappa Government Arts College, Karaikudi, Tamil Nadu, India

    Ramasamy Muthukrishnan Gobinath & Ramasubramanian Arumugam

  2. PG Department of Botany, Government Arts College, Melur, Madurai, Tamil Nadu, India

    Chinnathambi Pothiraj

  3. Department of Botany, V.H.N Senthikumara Nadar College (Autonomous), Virudhunagar, Tamil Nadu, India

    Periyasamy Periyakaruppiah

  4. Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia

    Daoud Ali & Saud Alarifi

  5. Centre for Microbial Technology, Department of Microbiology, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India

    Veeramani Veeramanikandan & Bhathini Vaikuntavasan Pradeep

  6. Centre for Herbal Pharmacology and Environmental Sustainability, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu, 603103, India

    Van-Huy Nguyen

  7. PG and Research Centre in Biotechnology, MGR College, Hosur, Tamil Nadu, India

    Paulraj Balaji

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  1. Ramasamy Muthukrishnan Gobinath
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Contributions

Conceptualization, RMG, CP and PB; methodology, RMG, RA; validation, PP, DA and SA; formal analysis, PP, DA, SA and PB; investigation, RMG, RA; data curation, VV, BVP and PB; Software: PP; writing—original draft preparation, RMG, RA and VHN; writing—review and editing, VV, VHN and PB; supervision, CP and PB.

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Correspondence to Chinnathambi Pothiraj, Van-Huy Nguyen or Paulraj Balaji.

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Gobinath, R.M., Pothiraj, C., Arumugam, R. et al. Biocatalytic Conversion of Lignocellulosic Water Hyacinth Biomass by Phanerochaete chrysosporium for Sustainable Ethanol Production. Top Catal (2024). https://doi.org/10.1007/s11244-024-01952-6

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