Abstract
A microponic system (MP system), a propagation system combines micropropagation and hydroponic that, has been studied on some flower and fruit plants; however, the MP system has not yet been tested on herbal plants, especially Jasminanthes tuyetanhiae (Apocynaceae, Asclepiadoideae). This study used the MP system with nylon film (A4 cover nylon) to evaluate rooting, abnormal developmental phenomena, antioxidant enzyme activities, secondary metabolites of plantlets, and subsequent growth. Results showed that 2-cm shoots cultured on a micropropagation system obtained a rooting rate of about 50–60%, and plantlets recorded abnormal phenomena reaching 85% consisting of 22.33% vitrification, 19.33% leaf abscission, 43.33% callus at the root and only 15% plantlets without abnormalities. In contrast, the MP system pre-treated with 0.5 mg/L indole butyric acid (IBA) and supplemented with 4 mg/L silver nanoparticles (AgNPs) obtained 100% rooting and improved plantlet quality. Furthermore, the plantlets developed using the MP system recorded a 2-fold reduction in vitrification and leaf abscission compared to those using the micropropagation system. A reduction in callus formation and increased antioxidant activities were also noted. The acclimatization of plantlets derived from the MP system pre-treated with 0.5 g/L IBA and 4.0 g/L AgNPs was higher than the conventional treatments.
Key message
Microponic system improved in vitro rooting and reduced abnormal phenomena (vitrification, leaf abscission, callus at the root) of Jasminanthes tuyetanhiae plantlet.
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Data availability
Data sharing does not apply to this article as no datasets were generated during the current study.
Abbreviations
- AgNPs:
-
Silver nanoparticles
- APX:
-
Ascorbate peroxidase
- BA:
-
6-benzylaminopurine
- CAT:
-
Catalase
- HPLC:
-
High-performance liquid chromatography
- IBA:
-
Indole-3-butyric acid
- MS:
-
Murashige and Skoog medium, 1962
- MSO:
-
MS medium supplemented with 30 g L− 1 sucrose, 0.5 mg L− 1 IBA and 7 g L− 1 agar
- MO:
-
Micropropagation system
- MP:
-
Microponic system
- ROS:
-
Reactive oxygen species
- SPAD:
-
Chlorophyll content of leaves checked by SPAD
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Acknowledgements
We thank Prof. Edward C. Yeung (University of Calgary, Canada) for reading and editing the manuscript.
Funding
Dr. Hoang Thanh Tung was funded by Vingroup JSC and supported by the Postdoctoral Scholarship Programme of Vingroup Innovation Foundation (VINIF), Institute of Big Data, code [VINIF.2022.STS.30].
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HTT acquired data and wrote the manuscript. NTNM, DMC, NBN, TTHT, NTV, HTMN, and BVTV participated in performing the experiments, interpretation of data, and revision for intellectual content. DTN and HTT conceptualized and designed the study. All authors discussed the results and contributed to the final manuscript.
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Tung, H.T., Mai, N.T.N., Cuong, D.M. et al. Enhanced rooting in in vitro derived shootlets of Jasminanthes tuyetanhiae T.B. Tran & Rodda (Apocynaceae, Asclepiadoideae) through microponic system. Plant Cell Tiss Organ Cult 157, 27 (2024). https://doi.org/10.1007/s11240-024-02753-1
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DOI: https://doi.org/10.1007/s11240-024-02753-1