Abstract
Taxodium hybrid ‘zhongshanshan’ is a valuable tree species with significant ecological and economic importance. However, successful conservation of its germplasm remains challenging. In this study, we explored and established a viable method for cryopreservation of embryogenic callus of Taxodium hybrid ‘zhongshanshan’. In the pretreatment process, sucrose was identified as the optimal pretreatment agent, with a concentration of 0.5 M and a treatment duration of 36 h resulting in the fast recovery of callus viability. Dimethyl sulfoxide (DMSO) played a crucial role in preserving cell viability in the freezing process. The most favorable recovery effect was observed with a concentration of 10% DMSO, which led to the shortest recovery period (5 days) and a high callus proliferation rate of 4.30. In the recovery process, thawing at 35 °C enabled the callus to regain viability within 3 days and achieve a proliferation rate of 6.39. Notably, the recovered callus exhibited no discernible differences in appearance compared to normally proliferated embryogenic callus, and maintained the ability to differentiate into somatic embryos. This study provided valuable insights for the effective preservation of Taxodium hybrid ‘zhongshanshan’ genetic resources, which can also be utilized for long-term conservation.
Key message
The present study successfully established an in vitro preservation method for germplasm resources of Taxodium hybrid ‘zhongshanshanshan’ for the first time.
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The original contributions presented in the study are included in the article, further inquiries can be directed to the corresponding author.
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Funding
This work was supported by Jiangsu Long-Term Scientific Research Base for Taxodium Rich. Breeding and Cultivation (LYKJ [2021]05) and the Jiangsu Institute of Botany Talent Fund (JIBTF202206).
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HJF contributed conception and design of the study; CTT and JXY performed the experiments; JXY, YCG and YYL carried out the statistical analysis; CTT wrote the manuscript. All authors contributed to the manuscript’s revision, read, and approved the submitted version.
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Communicated by Qiao-Chun Wang.
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Chen, T., Jia, X., Yu, C. et al. A simple and efficient protocol for cryopreservation of Taxodium hybrid ‘zhongshanshan’ embryogenic callus. Plant Cell Tiss Organ Cult 156, 44 (2024). https://doi.org/10.1007/s11240-023-02667-4
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DOI: https://doi.org/10.1007/s11240-023-02667-4