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Integration of phenylalanine precursor feeding and elicitor treatment in concert with the cellulase-assisted extraction approach to improve stilbene compound production

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Abstract

Trans-resveratrol, trans-arachidin-1 (Ara-1), and trans-arachidin-3 (Ara-3) are major stilbene compounds found in elicited peanut hairy root culture and exert diverse potential biological and pharmaceutical activities. The aim of this study was to investigate the various approaches for enhancing the productivity of prenylated stilbene. The fast-growing peanut hairy root K2-K599-C line was elicited with chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD) (CHT + MeJA + CD) as combined elicitors together with phenylalanine (Phe) as a precursor to feeding at 24, 48, and 72 h. The accumulation of stilbene compounds was investigated in this study as well as stilbene biosynthesis-related genes and plant defense gene expression. Upon an exogenous supply of Phe, elevated amounts of Ara-1 and Ara-3 were detected through the entire 72 h elicitation period while the non-Phe precursor feeding conditions exhibited an increasing amount of Ara-1 and Ara-3 up to 48 h, decreasing at 72 h. In addition, Phe precursor may serve as an early regulator of gene expression for the stilbene biosynthesis pathway, transcription factors, and pathogenesis-related proteins while a delayed response in transcriptional regulation was observed in the elicitation without Phe precursor feeding. Cellulase-assisted extraction was performed prior to solvent extraction of the culture medium. The results indicated a significant 1.73- and 1.57-fold increase in Ara-1 and Ara-3, respectively, compared to the non-cellulase-assisted process. To the best of our knowledge, this is the first study to propose an integrated approach to improving stilbene compound production in peanut hairy root culture.

Key message

An integrated approach of precursor feeding, elicitors, and enzyme-assisted extraction facilitates the improvement of stilbene compound production. Phenylalanine feeding induces early transcriptional regulation of plant response. Cellulase-assisted extraction improves the recovery of stilbene compounds.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by Agricultural Research Development Agency (Public Organization), Thailand 2022.

Funding

This study was funded by Agricultural Research Development Agency (Public Organization), Thailand 2022.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand

    Chonnikan Tothong, Chanyanut Pankaew, Phadtraphorn Chayjarung & Apinun Limmongkon

  2. Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand

    Anupan Kongbangkerd

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  1. Chonnikan Tothong
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  2. Chanyanut Pankaew
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  3. Phadtraphorn Chayjarung
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  4. Anupan Kongbangkerd
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  5. Apinun Limmongkon
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Contributions

CT: Conceptualization, Methodology, Validation, Formal analysis, Investigation. CP: Investigation, Formal analysis. PC: Validation, Investigation. AK: Resources, Visualization. AL: Conceptualization, Supervision, Visualization, Writing—Review & Editing.

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Correspondence to Apinun Limmongkon.

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Communicated by Christophe Hano.

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Tothong, C., Pankaew, C., Chayjarung, P. et al. Integration of phenylalanine precursor feeding and elicitor treatment in concert with the cellulase-assisted extraction approach to improve stilbene compound production. Plant Cell Tiss Organ Cult 155, 665–679 (2023). https://doi.org/10.1007/s11240-023-02586-4

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