Abstract
The present study is the first report on the quantitative analysis of secondary metabolites in callus cultures of Barleria prionitis L. and comparison with the mother plant. Callus was obtained from stem internode (on MS medium with 0.5 mg l−1 NAA, 0.5 mg l−1 BAP and 300 mg l−1 ascorbic acid), and leaf (on MS medium with 1.0 mg l−1 2,4-D, 0.5 mg l−1 BAP, 300 mg l−1 ascorbic acid ) explants and, multiplied by subculturing thrice at an interval of 25 days. Calli and mother plant counterparts were extracted in four solvents (methanol, ethanol, acetone, and distilled water) and examined for active compounds and antioxidant potential. Callus cultures not only preserved the mother plant’s metabolite profile but also displayed elevated levels. Leaf-derived callus surpassed stem-derived callus in most of the studied parameters. The highest total phenolic content (21.46 mg GAE g−1 FW) and total flavonoid content (24.58 mg of RE g−1 FW) were observed in methanol extract of leaf-derived callus, representing a 3-fold and 2-fold increase over mother plant leaf, respectively. Antioxidant capacity based on FRAP and DPPH assay was highest in methanol extract of leaf-derived callus (7-fold and 3-fold increase over mother plant, respectively) while ABTS activity was highest (122-fold increase) in acetone extract of leaf-derived callus. HPTLC analysis revealed enhanced concentrations of squalene (10-fold) and SME (2.3-fold) in acetone and methanol extract of leaf-derived callus, respectively, compared to mother explants. The results of RP-HPLC for phenolics showed the highest gallic acid content (99-fold increase) in ethanol extract of stem-derived callus whereas catechol was maximum (37-fold increase) in aqueous extract of leaf-derived callus. These findings suggest that callus cultures of B. prionitis L. can be a potential source of active metabolites. Further, cell suspension cultures can be produced from the callus, which could be an avenue for the large-scale production of bioactive compounds.
Key message
For the first time, active components of B. prionitis L. were determined quantitatively in callus cultures and compared with the mother plant.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author at a reasonable request.
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Acknowledgements
Authors are thankful to Dr. B.V. Ghule, Institute of Pharmaceutical Education and Research, Wardha, Maharashtra, India for providing guidance and laboratory facilities and SME standards for HPTLC. Support and resources provided by Dr. Debjani Dasgupta, Director, School of Biotechnology and Bioinformatics, D.Y. Patil Deemed to be University, Navi Mumbai are gratefully acknowledged.
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NJ: conception of the idea, experiment planning, interpretation of results and manuscript preparation, RR: conduction of experiments, and interpretation of results, SK: interpretation of results, data analysis and manuscript review.
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Communicated by Sergio J. Ochatt.
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Ranade, R., Joshi, N. & Kudale, S. Comparative secondary metabolite expression in callus cultures and mother plant in Barleria prionitis L.. Plant Cell Tiss Organ Cult 155, 653–663 (2023). https://doi.org/10.1007/s11240-023-02585-5
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DOI: https://doi.org/10.1007/s11240-023-02585-5