Abstract
A reliable and reproducible embryogenic cell suspension culture system established successfully for regeneration of plants using pro-embryogenic cell mass derived from secondary somatic embryos as explants. A semi-solid medium was employed, which consisted of Murashige and Skoog (MS) basal salts and vitamins supplemented with 4 mg l− 1 2,4-dichlorophenoxyacetic acid (2,4-D), 1 mg l− 1 biotin, 1 mg l− 1 indole acetic acid (IAA), 1 mg l− 1 naphthalene acetic acid (NAA), 25 mg l− 1 ascorbic acid, 3% (w/v) sucrose and 2 g l− 1 phytagel. The genetic integrity of the plants regenerated from the suspension cells was confirmed through various techniques, such as flow cytometry, simple-sequence repeats (SSR) markers and inter simple sequence repeats (ISSR) markers. The field planting displayed no phenotypic deviations and no adverse effects on the vegetative and yield characteristics in banana cv. Sabri, a rapidly declining landrace in Tripura, India due to severe Fusarium wilt incidence. Based on these insights, we propose research priorities and complementary strategies aimed at the efficient conservation and rejuvenation of such valuable landraces.
Key Message
The secondary somatic embryogenesis technique has demonstrated efficiency in establishing an embryogenic cell suspension culture system, offering great promise for producing genetically identical plants to rejuvenate banana cv. Sabri, a declining landrace.
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Funding
The authors acknowledge the Tripura Biotechnology Council, Govt of Tripura, India, for partial financial support. RK acknowledges Young Scientist Award received from SYST-SEED Division (SP/YO/124/2017(G)), DST, Govt of India.
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SU: administration, funding acquisition, supervision, validation. RK: conceptualization, visualization, investigation, methodology, formal analysis, data curation, writing—original draft—review & editing. SK: investigation, data curation, formal analysis, writing—review & editing. SB: supervision, formal analysis, writing—review & editing. MK: investigation, methodology, formal analysis, writing—review & editing. SS: investigation, methodology. MSS: investigation, methodology, supervision. PD: investigation, methodology. All authors read, and approved final version of manuscript.
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Uma, S., Karthic, R., Kalpana, S. et al. An efficient embryogenic cell suspension culture system through secondary somatic embryogenesis and regeneration of true-to-type plants in banana cv. Sabri (silk subgroup AAB). Plant Cell Tiss Organ Cult 155, 313–322 (2023). https://doi.org/10.1007/s11240-023-02570-y
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DOI: https://doi.org/10.1007/s11240-023-02570-y