Abstract
Water stress is one constraint of the development potential of many plant species under in vitro tissue culture. The present study aims to assess the responses of anise callus tissues to two concentrations (5 and 7.5%) of PEG-6000 under darkness and light (12 h-photoperiod) for 30 days. The exposure of calli to water stress recorded morphological and physiological variations in fresh weight and water content in the callus decreased with increasing dry weight. Malondialdehyde content increased progressively with concentrations used with higher values under dark than in light. The activity of antioxidant enzymes (SOD, CAT, APX, and GPOX) was affected by PEG-6000 concentrations and culture conditions. APX and CAT increased gradually with 5 and 7.5% of PEG-6000 with higher values under light, but SOD enzyme reacted differently with a slight increase with 5% in light and higher ones under darkness with 7.5%. Additionally, GPOX activity decreased with both concentrations independently of light or darkness. The results revealed that anise calli under drought stress with 7.5% PEG-6000 were more resistant to the lack of water in our experimental conditions.
Key message
The calli increased antioxidant enzyme activities, associated with water loss, lipid peroxidation, and decreased fresh weight in light/dark with PEG treatment. Tissue culture applications might offer solutions to water scarcity.
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Data availability
The experiment data generated and analysed during the current study are available from the corresponding author on request.
Change history
16 June 2023
The original version of this article has been revised: In the Discussion section, a duplicate heading and paragraph following the subsection ‘In vitro callus induction and growth’ were removed.
Abbreviations
- PEG:
-
Polyethylene glycol
- MDA:
-
Malondialdehyde
- CAT:
-
Catalase
- SOD:
-
Superoxide dismutase
- APX:
-
Ascorbate peroxidase
- GPOX:
-
Guaiacol peroxidase
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This work was supported by the Ministry of Higher Education and Scientific Research (Algeria). No fund, grant were received. We express our gratitude for all members of both laboratories for their help.
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CD and DR were contributed to the study conception and design. CD, DR, LS and BL carried out experiments Callus establishment without and with PEG treatment were performed by LS and CD Physiological and enzymes activities were carried out by LS and BL with DR. All authors contributed to data analysis, reading and approved final manuscript.
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Communicated by Patricia Marconi.
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Lamara, S., Boucelha, L., Djebbar, R. et al. Antioxidant activities in Pimpinella anisum L. callus responding to PEG-6000-induced water stress under light (12 h-photoperiod) and dark conditions. Plant Cell Tiss Organ Cult 154, 399–413 (2023). https://doi.org/10.1007/s11240-023-02530-6
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DOI: https://doi.org/10.1007/s11240-023-02530-6