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Cloning and functional characterization of MhPSY1 gene from Malus halliana in apple calli and Arabidopsis thaliana

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Abstract

Salinization is a universal problem in the world, which seriously affects the growth and development of plants, limit the quality and yield. Additionally, the study of stress-related genes and their regulatory molecular mechanisms is of great importance to cope with saline-alkali stress. In this study, based on transcriptome of Malus halliana, we screened out the gene Phytoene synthase 1 (PSY1, LOC103405286) significantly induced by saline-alkali stress in carotenoid metabolic pathways. Quantitative real-time PCR confirmed that the expression level of MhPSY1 was significantly down-regulated in the leaves of M. halliana under saline-alkali conditions. Subsequently, MhPSY1 gene was isolated and its functional characteristics were further studied in Arabidopsis thaliana and apple calli. Bioinformatics analysis found that MhPSY1 contains a complete open reading frame with a length of 1191 bp and encodes 396 amino acids with an isoelectric point of 9.06, which is an alkaline protein. Phylogenetic tree analysis showed that MhPSY1 had the highest homology and the closest genetic relationship with Pyrus×bretschneideri. Stress resistance test showed that overexpression of MhPSY1 reduced the tolerance of Arabidopsis thaliana and apple calli to saline-alkali stress and the main basis is as follows: the SOD, POD and CAT activities of overexpressed apple calli and transgenic Arabidopsis were significantly lower than those of wild type (WT), malondialdehyde (MDA) content was significantly higher than that of WT and proline content was lower than that of WT. Meanwhile, phenotypic observations found that the growth of transgenic plants was inhibited under saline-alkali stress. In conclusion, MhPSY1 gene involved in carotenoid metabolic pathway plays a negative regulatory role in saline-alkali stress.

Key message

The gene MhPSY1, which was significantly expressed under saline-alkali stress, was cloned andtransformed into Arabidopsis and apple calli, and it was found that MhPSY1 gene negativelyregulated salt-alkali stress

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Data Availability

All data generated or analysed during this study are included in this published article.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (Project Number 31960581).

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Authors and Affiliations

  1. Horticulture College of Gansu Agricultural University, Lanzhou, China

    Yuxia Wu, Shuangcheng Wang, Wanxia Wang, Xiu Wang, Yongjuan Dong, Cong Han & Yanxiu Wang

Authors
  1. Yuxia Wu
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  2. Shuangcheng Wang
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  3. Wanxia Wang
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  4. Xiu Wang
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  5. Yongjuan Dong
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  6. Cong Han
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  7. Yanxiu Wang
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Contributions

YX Wu and YX Wang designed the research. YX Wu, SC Wang and WX Wang performed the experiments. X Wang and C Han performed the data analysis and interpretation. SC Wang and YJ Dong prepared the figures and tables. YX Wu wrote the manuscript. All authors read, commented on and approved the manuscript.

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Correspondence to Yanxiu Wang.

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Communicated by Henryk Flachowsky.

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Wu, Y., Wang, S., Wang, W. et al. Cloning and functional characterization of MhPSY1 gene from Malus halliana in apple calli and Arabidopsis thaliana. Plant Cell Tiss Organ Cult 154, 249–259 (2023). https://doi.org/10.1007/s11240-023-02501-x

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  • DOI: https://doi.org/10.1007/s11240-023-02501-x

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