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Improved antioxidant metabolism in shoot cultures of Ruta graveolens (L.) in response to methyl jasmonate and abscisic acid

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Abstract

Polyphenolic compounds are an important class of plant secondary metabolites known to exhibit high antioxidant properties. Plant tissue cultures have been an attractive alternate source of such compounds and their biosynthesis can be improved by elicitation. The present study evaluated the effect of phytohormone elicitors viz. methyl jasmonate and abscisic acid on antioxidant components in in vitro shoot cultures of Ruta graveolens L., a well explored medicinal plant. Phytohormones were added into the culture medium and the shoot cultures were harvested after 5, 10 and 15 days post elicitation for analyses. Elicitor type and exposure time significantly influenced each analyzed parameter. Highest content of total phenolic compounds (TPC) was recorded in shoots exposed to methyl jasmonate for 5 days (44.33 µg Gallic acid equivalent g− 1 FW), representing a 4.4- fold increase compared to control. Highest total flavonoid content (TFC) (6.6-fold increase; 1.43 µg Quercetin equivalent g− 1 FW) and total flavonol (TFL) (2-fold increase; 1.22 mg Rutin equivalent g− 1 FW) was recorded in shoots treated with abscisic acid for 5 and 15 days, respectively. Phenyl ammonia lyase (PAL) activity was in correspondence with TPC and TFC in shoots treated with both the elicitors. The antioxidant potential as revealed by DPPH and FRAP assay was highest in shoots elicited with abscisic acid (2-fold increase, 10th day) and methyl jasmonate (1.5- fold increase, 5th day), respectively. Results of reverse phase high performance liquid chromatography (RP-HPLC) revealed MJ as the better elicitor for production of phenolics. This study demonstrates the potential of elicitation strategy for enhanced accumulation of polyphenolic compounds in shoot cultures of R. graveolens.

Key message

Methyl jasmonate and abscisic acid increased antioxidant metabolite production in shoot cultures of R. graveolens without compromising on the growth. Exposure time of MJ and ABA significantly influenced the metabolite synthesis.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

Support and facilities provided by Dr. Debjani Dasgupta, Director, School of Biotechnology and Bioinformatics, D.Y. Patil Deemed to be University, Navi Mumbai is gratefully acknowledged.

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Authors and Affiliations

  1. School of Biotechnology and Bioinformatics, D.Y. Patil Deemed To be University, Plot No. 50, Sector-15, CBD Belapur, Navi Mumbai, 400614, India

    Neelu Joshi, Kirti Agarwal & Sourav Ghosh

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  1. Neelu Joshi
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  2. Kirti Agarwal
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  3. Sourav Ghosh
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Contributions

N. Joshi, K. Agarwal contributed in conduct of experiments and interpretation of results. N. Joshi and S. Gosh contributed in interpretation of results, data analysis and preparation of manuscript.

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Correspondence to Neelu Joshi.

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Communicated by Pamela J. Weathers.

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Joshi, N., Agarwal, K. & Ghosh, S. Improved antioxidant metabolism in shoot cultures of Ruta graveolens (L.) in response to methyl jasmonate and abscisic acid. Plant Cell Tiss Organ Cult 153, 367–376 (2023). https://doi.org/10.1007/s11240-023-02474-x

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