Abstract
In this work, the impact of microgravity analog (clinorotation) induced by a 2D-clinostat was investigated on the growth, chlorophyll (Chl) metabolism, enzyme activities, and antioxidant metabolites of Ocimum basilicum. Clinorotation was applied at two acceleration levels (Clino 3 (0.013 g) and Clino 2 (0.009 g)) and ground control (1 g) at the early growth stage for detection of the potential threshold acceleration of plant perception. Results showed that fresh weight, root and shoot length, adventitious roots, Chl pigments, and their precursors were enhanced in the seedlings grown under clinorotation, especially Clino 2 compared to the control. Increased content of Chl pigments and their precursors were positively related to the more induction of Chl fluorescence, aminolevulinic acid, and dehydratase enzyme activity with decreasing acceleration. Clinorotation, especially under Clino 3 enhanced markedly superoxide radical content while decreased the content of heme. The activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase increased in O. basilicum seedlings which were associated negatively with the reduction of heme content. Rubisco gene expressions (rbcL and rbcS) were upregulated and led to the induction of Rubisco activity. Total carbohydrate and protein contents increased in clinorotated seedlings, but free amino acids did not change compared to the control. Moreover, antioxidant compounds such as phenolics, flavonoids, anthocyanin, and carotenoids increased with decreasing acceleration in one-week clinorotated seedlings. These findings display the impact of microgravity analog on the more function of chloroplast (Chl metabolism and fluorescence, and Rubisco) at the early stage of growth and will help to improve ground-based studies leading to space experiments.
Key message
The potential threshold acceleration for the gravity-dependent growth response was obtained in the lower g-force at the initial vegetative stage. Clinorotation by up-regulation of rubisco gene expression and activity and Chl metabolism promoted photosynthetic performance for growth induction.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Authors give thanks to the Paya Kesht Company for preparing the clinorotation vehicle.
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HH performed the experiments, designed, and supervised the entire work. AAHAL performed the necessary statistical analysis. Both authors read and approved the manuscript.
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Hassanpour, H., Abdel Latef, A.A.H. Changes in chlorophyll metabolism, Rubisco gene expression, and antioxidant metabolites of Ocimum basilicum seedlings subjected to clinorotation. Plant Cell Tiss Organ Cult 153, 119–132 (2023). https://doi.org/10.1007/s11240-023-02448-z
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DOI: https://doi.org/10.1007/s11240-023-02448-z