Abstract
Numerous complex chemicals are being routinely used to prevent pathogens in various commercially important agri-horticultural crops that have some biosafety issues associated with human health and environment. Therefore, the present study was aimed to develop resistance in tomato against soil-borne fungal pathogen Fusarium oxysporum f. sp. lycopersici, through in vitro cell line selection approach. BLASTn analysis of sequenced 16S rRNA region of the fungus isolated from the wilt infected tomato plant was confirmed by ITS gene technology. Highest callus induction (79.63 ± 0.70%) along with shoot regeneration (78.68 ± 0.64%) was recorded on MS medium fortified containing 4.54 µM TDZ + 2.90 µM IAA and 2.27 µM TDZ + 1.0 µM 2,4-D using leaf explants, respectively. To carry out in vitro selection, developed calli using leaf explants were further subjected on selective medium followed by discontinuous selection cycles supplemented with 30% culture filtrate (CF) of targeted pathogen, where 21.32 ± 0.99 per cent average callus survival and 45.98 ± 0.55% average shoot induction (devoid of CF) were recorded. 12 moderately resistant lines were obtained from the total 48 cell lines during in vivo bioassay under glass house conditions. Two somaclones were confirmed at molecular level among the total 12 moderately developed resistant cell lines. The multilocation field trials are being done to check stable inheritance with generation wise advancements. It was inferred from the present study that these findings could be a cutting edge to develop resistance against various stresses in commercially important horticultural crops like tomato without posing any threat to environment.
Key message
Two tolerant tomato somaclones developed against Fusarium oxysporum f. sp. lycopersici causing Fusarium wilt disease using an ecofriendly research methodology of in vitro cell line selection approach.
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Acknowledgements
The authors are grateful to the Department of Environment, Science & Technology, Shimla (HP) India for funding the present research work.
Funding
This work was supported by Department of Environment, Science & Technology (DEST), Shimla (HP) India (No. Env.S&T(F)/R&D Project/2017dated 09/02/2018). Author Rajnish Sharma has received research support from DEST, Shimla (HP) India.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by IS, RS, PS, MG and RKB. The first draft of the manuscript was written by IS, RS and PS. All authors read and approved the final manuscript.
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Shaunak, I., Sharma, R., Sharma, P. et al. Developing resistance against soil-borne Fusarium pathogen causing tomato wilt through in vitro cell line selection. Plant Cell Tiss Organ Cult 153, 91–104 (2023). https://doi.org/10.1007/s11240-023-02446-1
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DOI: https://doi.org/10.1007/s11240-023-02446-1