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Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor

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Abstract

Brassavola nodosa (L.) Lindl. is a tropical epiphytic orchid showing characteristics of interest for the ornamental nursery industry, as well as considered as an endangered species. Problems with traditional propagation methods limit the development of a large-scale commercial production system. The use of micropropagation has been investigated for this species, and the use of liquid in vitro systems showed potential for use of temporary immersion systems, also called temporary immersion bioreactors (TIB) for micropropagation of B. nodosa. This study evaluated the efficiency of the SETIS™ TIBs for the micropropagation of B. nodosa. Explants consisting of young shoots tips measuring between 0.3 and 0.5 cm were collected from 1-yr-old in vitro seedlings of B. nodosa and used for shoot multiplication. Four culture systems were evaluated: a semi-solid in vitro system consisting of 160 mm × 100 mm TP1600 Microbox containing 500 ml semi-solid medium as a control, and a liquid in vitro system consisting of 4 L temporary immersion bioreactor (SETIS™, VERVIT, Belgium) containing 500 ml liquid medium with immersion and aeration frequencies of 2 h, 4 h, and 8 h. For all bioreactor treatments, duration of immersion and aeration was 2 min. Results showed that temporary immersion of B. nodosa explants with a frequency of 2 h and duration of 2 min returned the highest multiplication rates, with 4.6 shoots produced per explant compared with 2.8 shoots per explant in semi-solid agar-based systems. The use of bioreactors also promoted increased growth and development and in vitro rooting, therefore improving survival, and facilitating acclimatization of in vitro-derived plantlets. This is the first study demonstrating a successful protocol for micropropagation of B. nodosa using SETIS™ bioreactors, which could have significant value and impact for the commercial production of this species as well as for conservation purposes.

Key message

Micropropagation of Brassavola nodosa was achieved using SETIS temporary immersion bioreactors, showing good multiplication rates, increased growth and development and in vitro rooting, thus improving survival and acclimatization ex vitro.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work is supported by the USDA National Institute of Food and Agriculture, Hatch project 1012202.

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The authors declare that no funds, Grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Environmental Horticulture Department, University of Florida, Gainesville, FL, USA

    Wagner A. Vendrame, JianJian Xu & David G. Beleski

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  1. Wagner A. Vendrame
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  2. JianJian Xu
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  3. David G. Beleski
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by JX, and DB. The first draft of the manuscript was written by WV and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wagner A. Vendrame.

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Communicated by Jerico Jabin Bello Bello.

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Vendrame, W.A., Xu, J. & Beleski, D.G. Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor. Plant Cell Tiss Organ Cult 153, 67–76 (2023). https://doi.org/10.1007/s11240-022-02441-y

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