Abstract
Cereus hildmannianus (Cactaceae) is a medicinal plant with various medicinal and industrial applications. Plant biotechnology is an attractive approach for specialized metabolite production under controlled conditions, with the elicitation process being considered a highly effective strategy for increasing the production of bioactive compounds. In this regard, the present study investigated the effects of elicitors, sucrose (S, 1–8 g L−1), UV-C light (UV-C, 10–80 min), salicylic acid (SA, 50–200 µmol), and jasmonic acid (JA, 50–200 µmol), on the profile of esterase isozymes, total phenolic and total flavonoid contents, antioxidant activity (FRAP, DPPH, and ABTS), and the dereplication method by Ultra-High-Performance Liquid Chromatography coupled with Mass Spectrometry (UHPLC-ESI-Q-TOF–MS/MS). The isozyme profile was not significantly changed. The highest accumulations of total phenolics were observed in callus tissues induced with 100 µmol JA (390.8 µg GAE mg−1 DW), 200 µmol SA (355.5 µg GAE mg−1 DW), 20 min UV-C (182.9 µg GAE mg−1 DW), and 6 g L−1 S (122.1 µg GAE mg−1 DW); the highest concentrations of total flavonoids were observed following culture with 200 µmol SA (89.8 µg QE mg−1 DW) and 2 g L−1 S (28.4 µg QE mg−1 DW). Furthermore, the dereplication study allowed putative identification of thirty-six compounds (thirty phenolic acids and organic acids, and six flavonoids). The highest antioxidant activities were obtained with callus tissues induced with 100 µmol JA, 200 µmol SA, 80 min UV-C, and 1 g L−1 S. The elicitors were able to alter the production of phenolic compounds in callus cultures of C. hildmannianus. These results are promising for the clean and sustainable bioproduction of bioactive molecules for pharmaceutical and cosmeceutical purposes, prioritizing the conservation of the species.
Key message
Elicitation of callus tissues of Cereus hildmannianus with sucrose, UV-C light, salicylic acid, and jasmonic acid enhances the phenolic compound content with antioxidant activity, which is known to be associated with promising antiaging activity.
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Abbreviations
- DW:
-
Dry weight
- JA:
-
Jasmonic acid
- MS:
-
Murashige and Skoog medium
- PPFD:
-
Photosynthetic photon flux density
- S:
-
Sucrose
- SA:
-
Salicylic acid
- TFC:
-
Total flavonoid content
- TPC:
-
Total phenolic content
- UHPLC-ESI-Q-TOF–MS/MS:
-
Ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
- UV-C:
-
Ultraviolet light C
- UV–Vis:
-
Ultraviolet–visible spectroscopy
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The authors would like to thank the National Council for Scientific and Technological Development, Coordination for the Improvement of Higher Education Personnel [CAPES Finance Code 001], and Fundação Araucária for financial support.
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All authors approve of the version to be submitted. ÉSS, MRPC, TFOS, AJBO, MFPSM, and CAM: performed the study and examined the experimental data. ÉSS: prepared the manuscript. RACG: participated in the interpretation of data and revision for important intellectual content and completed the final version of the manuscript.
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da Silva Santos, É., Cabral, M.R.P., da Silva, T.F.O. et al. Improvement of phenolic compounds production in callus cultures of Cereus hildmannianus (K.) Schum. by elicitation. Plant Cell Tiss Organ Cult 153, 37–51 (2023). https://doi.org/10.1007/s11240-022-02438-7
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DOI: https://doi.org/10.1007/s11240-022-02438-7