Abstract
The multiplication of sugarcane (Saccharum spp.) variety LAICA 04-809 by direct organogenesis was evaluated using four cultivation systems: (1) the traditional system which uses a semisolid medium and (2) three temporary immersion systems with liquid medium (RITA®, BIT®, and SETIS™). In addition, three planting densities (10, 30, and 50 mL of medium per explant) were studied for each system as initial inoculum. Sucrose consumption was observed in all temporary immersion systems after 4 weeks of culture. Photomixotrophic metabolism was identified in all three temporary immersion systems evaluated (RITA®, BIT®, and SETIS™). In addition, at density of 30 mL medium/explant, the BIT® bioreactor generated the highest number of quality shoots (425.33 ± 24.58). No hyperhydricity was observed in the explants. In TISs, the 50 mL volume of medium per explant obtained the highest multiplication coefficient (RITA® 25.53 ± 11.42, BIT® 20.08 ± 4.1 and SETIS™ 11.47 ± 1.27). The results of this study suggest that the combination of the BIT® system with the 50 mL volume of medium/explant represents the best conditions for mass propagation of sugarcane shoots after four weeks of cultivation.
Key message
The response of in vitro multiplication of sugarcane depends on the type TISs and the planting density (mL of media per explant). Higher quality of shoots was obtained with BIT®.
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Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We acknowledge Javier Alfaro and Erick Chavarría of Dirección de Investigación y Extensión de la Caña de Azúcar (DIECA) in Liga Agrícola Industrial de la Caña de Azúcar (LAICA, Costa Rica) for providing in vitro sugarcane plants variety LAICA 04-809 used in this research.We also acknowledge Diego Rojas Gätjens and Efraín Escudero Leyva of Centro Nacional de Innovaciones Biotecnológicas (CENIBiot) for their contributions in this research.
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This research was funded by Centro Nacional de Alta Tecnología and Centro Nacional de Innovaciones Biotecnológicas (CENIBiot-CeNAT).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by COO, LS and JAM. The first draft of the manuscript was written by COO, IVS and EAV and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Orozco-Ortiz, C., Sánchez, L., Araya-Mattey, J. et al. BIT® bioreactor increases in vitro multiplication of quality shoots in sugarcane (Saccharum spp. variety LAICA 04-809). Plant Cell Tiss Organ Cult 152, 115–128 (2023). https://doi.org/10.1007/s11240-022-02392-4
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DOI: https://doi.org/10.1007/s11240-022-02392-4