Abstract
Micropropagation is a promising technique for hop (Humulus lupulus L.) plantlet production. However, the phenotypic stability of micropropagated hop plants is poorly understood. These should be characterized, in order to identify possible variations. The purpose of this study was to analyze and characterize the occurrence of somaclonal variation induced by in vitro multiplication of hop genotypes. Three genotypes were established in vitro and micropropagated in five subcultures. The response variables were chosen by the stepwise method and subjected to multivariate analysis of variance (Manova), multivariate contrasts, standardized canonical coefficients (StCC) and intraclass correlation (t). Somaclonal variation was detected in the genotypes Chinook and Columbus, both from America. No significant variations were found in the European genotype Hallertauer Mittelfrüeh, regardless of the number of subcultures.
Key message
Somaclonal variation was detected in the genotypes Chinook and Columbus, both from America. No significant variations were found in the genotype Hallertauer Mitterlfrüeh, regardless of the number of subcultures.
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Acknowledgements
The authors are indebted to the State University of Santa Catarina (UDESC, Brazil), National Council for Scientific and Technological Development (CNPq), Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) and Foundation for the Support of the Scientific and Technological Research of Santa Catarina (FAPESC) for the scholarship and funding of this research.
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This study was funded by National Council for Scientific and Technological Development (CNPq) and Foundation for the Support of the Scientific and Technological Research of Santa Catarina (FAPESC).
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Each author contributed individually and significantly to the development of this study. Conceptualization: JLMC; RS; AFG; AM; Data acquisition: RS; CRA. Data analysis: JLMC; RS; RCM; Writing and editing: RS; RCM; CRA. AM; AFG.
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de Souza, R., Adams, C.R., de Melo, R.C. et al. Phenotypical changes in hop induced by micropropagation. Plant Cell Tiss Organ Cult 147, 379–387 (2021). https://doi.org/10.1007/s11240-021-02130-2
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DOI: https://doi.org/10.1007/s11240-021-02130-2