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Analysis of Chinese Spring regenerants obtained from short- and long-term wheat somatic embryogenesis

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Summary

Somatic embryogenesis was initiated from ‘immature embryos’ on Murashige-Skoog (MS) medium plus 2 mg.l-1 2,4-dichlorophenoxyacetic acid, 2% sucrose and 0.6% agarose. Somatic embryos were isolated and regenerated into whole green plants on MS medium devoid of 2,4-D. These regenerants were previously demonstrated to differ in their mitochondrial DNA organization. In order to estimate their characteristics three progenies of short-term culture regenerants and three progenies of long-term culture regenerants were analyzed and compared to the parental line. These somaclones obtained from the wheat variety Chinese Spring were evaluated for variation of 13 agronomic and morphological quantitative characters in comparison to the parental line. Significant variation was observed for plant height, spike length, main tiller diameter, between the somaclones regenerated from long-term culture and their parent. Differences were observed to increase with the duration of culture, leading to a significant modification of the structure of the plants. Several changes occurred during the somatic tissue cultures, but to a lesser extent than has previously been described in the literature.

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References

  • Armstrong, K.C., C., Nakamura & W.A., Keller, 1983. Karyotype instability in tissue culture regenerants of Triticale (× Triticosecale Wittmack) cv. ‘Welsh’ from 6-month-old callus culture. Z. Pflanzenzüchtg., 91: 233–245.

    Google Scholar 

  • Brears, T., G.J., Curtis & D.M., Lonsdale, 1989. A specific rearrangement of mitochondrial DNA induced by tissue culture. Theor. Appl. Genet. 77: 620–624.

    Google Scholar 

  • Breiman, A., T., Felsenburg & E., Galun, 1989. Is Nor region variability in wheat invariably caused by tissue culture? Theor. Appl. Genet. 77: 809–814.

    Google Scholar 

  • Brettell, R.I.S., E.S., Dennis, W.R., Scowcroft & W.J., Peacock, 1986. Molecular analysis of a somaclonal mutant of maize alcohol dehydrogenase. Mol. Gen. Genet. 202: 235–239.

    Google Scholar 

  • Brettell, R.I.S. & E.S., Dennis, 1991. Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern. Mol. Gen. Genet. 229: 365–372.

    Google Scholar 

  • Brown, P.T.H., 1989. DNA methylation in plants and its rôle in tissue culture. Genome 31: 717–729.

    Google Scholar 

  • Brown, P.T.H., J., Kyozuka Y., Sukekiyo, Y., Kimura, K., Shimamoto & H., Lörz, 1990. Molecular changes in protoplast-derived rice plants. Mol. Gen. Genet. 223: 324–328.

    Google Scholar 

  • Carver, B.F. & B.B., Johnson, 1989. Partitioning of variation derived from tissue culture of winter wheat. Theor. Appl. Genet. 78: 405–410.

    Google Scholar 

  • Charmet, G., S., Bernard & M., Bernard, 1986. Origin of aneuploid plants obtained by anther culture in triticale. Can. J. Genet. Cytol. 28: 444–452.

    Google Scholar 

  • Day, A. & T.H.N., Ellis, 1984. Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplasts. Cell 39: 359–368.

    Google Scholar 

  • De, Buyser, J., Y., Henry & G., Talab, 1985. Wheat androgenesis: cytogenetical analysis and agronomic performance of doubled haploids. Z. Pflanzenzüchtg. 95: 23–34.

    Google Scholar 

  • De, Buyser, J., C., Harmann, Y., Henry, & A., Rode, 1988. Variation in long-term wheat somatic tissue cultures: chromosomal number, plant morphology and mitochondrial DNA. Can. J. Bot. 66: 1891–1895.

    Google Scholar 

  • Gengenbach, B.G. & J.A., Connelly, 1981. Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor. Appl. Genet. 59: 161–167.

    Google Scholar 

  • Gould, A.R., 1982. Chromosome instability in plant tissue cultures studied with banding techniques. In: A. Fujiwara (Ed.) Plant Tissue culture 1982. Proc. 5th Congress Intl. Ass. for Plant Tissue and Cell Culture: 431–432.

  • Grisvard, J., 1985. Different methylation pattern of melon satellite DNA sequences in hypocotyl and callus tissues. Plant Science 39: 189–193.

    Google Scholar 

  • Grisvard, J., M., Sevignac, M., Chateau & M., Branchard, 1990. Changes in a repetitive DNA sequence during callus culture of Cucumis melo. Plant Science 72: 81–91.

    Google Scholar 

  • Hartmann, C., Y., Henry, J., De, Buyser, C., Aubry & A., Rode, 1989. Identification of new mitochondrial genome organization in wheat plants regenerated from somatic tissue culture. Theor. Appl. Genet. 77: 169–175.

    Google Scholar 

  • Henry, Y. & J., De, Buyser, 1990. Wheat anther culture: Agronomic performance of doubled haploid lines and the release of a new variety ‘Florin’. In: Y.P.S., Bajaj (Ed.) Biotechnology in Agriculture, vol. 13: Wheat, pp. 285–352 Springer-Verlag Berlin.

    Google Scholar 

  • Hirochika, H., 1993. Activation of tobacco retrotransposons during tissue culture. Embo J. 12: 2521–2528.

    Google Scholar 

  • James, M.G. & J., Stadler, 1989. Molecular characterization of mutator systems in maize embryogenic callus cultures indicates Mu element activity in vitro. Theor. Appl. Genet 77: 383–393.

    Google Scholar 

  • Kaeppler, S.M. & R.L., Phillips, 1993. Tissue culture-induced DNA methylation variation in maize. Proc. Natl. Acad. Sci. USA 90: 8773–8776.

    Google Scholar 

  • Karp, A. & S.E., Maddock, 1984. Chromosome variation in wheat plants regenerated from cultured immature embryos. Theor. Appl. Genet. 67: 249–255.

    Google Scholar 

  • Karp, A., Q.S., Wu, S.H., Steele & M.G.K., Jones, 1987. Chromosome variation in dividing protoplasts and cell suspension of wheat. Theor. Appl. Genet. 74: 140–146.

    Google Scholar 

  • Kaul, M.L.H. 1988. Male sterility in higher plants, Springer-Verlag Berlin, 1005 p.

    Google Scholar 

  • Kemble, R.J. & J.F., Shepard, 1984. Cytoplasmic DNA variation in potato protoclonal population. Theor. Appl. Genet. 69: 211–216.

    Google Scholar 

  • Kidwell, K.K. & T.C., Osborn, 1993. Variation among alfalfa somaclones in copy number repeated DNA sequences. Genome 36: 906–912.

    Google Scholar 

  • Lipitan, N.L.V., R.G., Sears & B.S., Gill 1984. Translocations and other karyotypic structural changes in wheat x rye hybrids regenerated from tissue culture. Theor. Appl. Genet. 68: 547–554.

    Google Scholar 

  • Lapitan, N.L.V., R.G., Sears & B.S., Gill, 1988. Amplification of repeated DNA sequences in wheat x rye hybrids regenerated from tissue culture. Theor. Appl. Genet. 75: 381–388.

    Google Scholar 

  • Lee, M. & R.L., Phillips, 1988. The chromosomal basis of somaclonal variation. Ann. Rev. Plant Physiol. Plant. Mol. Biol. 39: 413–437.

    Google Scholar 

  • Li, X.Q., P., Chetrit, C., Mathieu F., Vedel, R., De, Paepe, R., Remy & F., Ambard-Bretteville, 1988. Regeneration of cytoplasmic male sterile protoclones of Nicotiana sylvestris with mitochondrial variations. Current Genetics 13: 261–266.

    Google Scholar 

  • MacCoy, T.J., R.L., Phillips & H.W., Rines, 1982. Cytogenetic analysis of plants regenerated from oat (Avena sativa) tissue cultures: high frequency of partial chromosome loss. Can. J. Genet. cytol. 24: 37–50.

    Google Scholar 

  • Maddock, S.E., 1985. Cell culture, somatic embryogenesis and plant regeneration in wheat, barley oats, rye and triticale. In: S.W.J., Bright & M.G.K., Jones (Eds) Cereal tissue and cell culture, pp. 131–174 Kluwer Academic Publishers, Lancaster.

    Google Scholar 

  • Maddock, S.E. & J.T., Semple, 1986. Field assessment of somaclonal variation in wheat. J. Exp. Bot. 37: 1065–1078.

    Google Scholar 

  • Metakovsky, E.V., A., Yu, Novoselskaya & A.A., Sozinov, 1987. Problems of interpreting results obtained in studies of somaclonal variation in glidin proteins in wheat. Theor. Appl. Genet. 73: 764–766.

    Google Scholar 

  • Mohmand, A.S. & M.W., Nabors, 1990. Somaclonal variant plants of wheat derived from mature embryo explants of three genotypes. Plant Cell Reports 8: 558–560.

    Google Scholar 

  • Müller, E., P.T.H., Brown, S., Hartke & H., Lörz, 1990. DNA variation in tissue culture-derived rice plants. Theor. Appl. Genet. 80: 673–679.

    Google Scholar 

  • Murashige, T. & F., Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Pl 15: 473–497.

    Google Scholar 

  • Orton, T.J., 1980. Chromosomal variability in tissue cultures and regenerated plants of Hordeum. Theor. Appl. Genet. 56: 101–112.

    Google Scholar 

  • Peschke, V.M., R.L., Phillips & B.G., Gengenbach, 1987. Discovery of transposable element activity among progeny of tissue culturederived maize plants. Science 238: 804–807.

    Google Scholar 

  • Peschke, V.M., & R.L., Phillips 1991. Activation of the maize transposable element Suppressor-mutator (Spm) in tissue culture. Theor. Appl. Genet. 81: 90–97.

    Google Scholar 

  • Prem Das, O., S., Levi-Minzi M., Koury, M., Benner & J., Messing, 1990. A somatic gene rearrangement contributing to genetic diversity in maize. Proc. Natl Acad. Sci. USA 87: 7809–7813.

    Google Scholar 

  • Rode, A., C., Hartmann, D., Falconet, B., Lejeune, F., Quetier, A., Banslimane, Y., Henry & J., De, Buyser, 1987. Extensive mitochondrial DNA variation in somatic tissue cultures initiated from wheat immature embryos. Current Genetics 12: 369–376.

    Google Scholar 

  • Shirzadegan, M., M., Christey, E.D., Earle & J.D., Palmer, 1989. Rearrangement, amplification and assortment of mitochondrial DNA molecules in cultured cells of Brassica campestris. Theor. Appl. Genet. 77: 17–25.

    Google Scholar 

  • Snape, J.W., L.A., Sitch, E., Simpson & B.B., Parker, 1988. Tests for the presence of gametoclonal variation in barley and wheat doubled haploids produced using the Hordeum bulbosum system. Theor. Appl. Genet. 75: 509–513.

    Google Scholar 

  • Worland, A.J., M.D., Gale & C.N., Law, 1988. Wheat genetics. In: F.G.H., Lupton (Ed.) Wheat Breeding, Chapman & Hall London: 129–171.

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Génétique Végétale, Bt 360, URA CNRS 115, Université Paris XI, 91405, Orsay, France

    Y. Symillides, Y. Henry & J. De Buyser

  2. Plant Breeding and Biometry, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Y. Symillides

  3. Laboratoire de Biologie Moléculaire Végétale, Bt 430, URA CNRS 1128, Université Paris XI, 91405, Orsay, France

    Y. Henry & J. De Buyser

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  1. Y. Symillides
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  2. Y. Henry
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  3. J. De Buyser
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Symillides, Y., Henry, Y. & De Buyser, J. Analysis of Chinese Spring regenerants obtained from short- and long-term wheat somatic embryogenesis. Euphytica 82, 263–268 (1995). https://doi.org/10.1007/BF00029569

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  • DOI: https://doi.org/10.1007/BF00029569

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