Abstract
The cellular and biochemical features associated with expression of pluripotency in endosperm cells during de novo shoot organogenesis, as well as the cytogenetic and morpho-agronomic features of regenerated Passiflora foetida triploid plants has been recently reported. Endosperms were cultured on Murashige and Skoog medium supplemented with 0.5, 0.75, 1.0, 1.5, and 2.0 mg L−1 of 6-benzyladenine, thidiazuron, or kinetin. No plant growth regulators were added to the control treatments. Morphogenetic responses were observed only in the presence of cytokinins, especially thidiazuron. The highest percentage of morphogenetic responses (27%) and number of adventitious shoots per explant (68.2) were observed with 1.5 and 2.0 mg L−1 thidiazuron, respectively. The regeneration of adventitious shoots occurred mostly from the outer face of the endosperm by cells that acquired meristematic-like features. Lipids and proteins were consumed rapidly, whereas carbohydrates increased considerably throughout the organogenic process. Cytogenetic analyses confirmed the triploid genotype of endosperm-derived plants, whose vegetative and floral structures were nearly all larger than their diploid counterparts. This study provides a complete morphological characterization of P. foetida endosperm-derived triploid plants and opens new possibilities for genetic breeding of passion fruit.
Key Message
Triploid plants of Passiflora foetida originating from in vitro endosperm culture are morphologically distinct from their diploid counterparts.
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Acknowledgements
This work was supported by the Fundação de Amparo à Pesquisa do Estado de Mato Grosso (FAPEMAT) (Cuiabá, MT), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (Belo Horizonte, MG; Grant APQ-00772-19), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) – (Brasília, DF; Finance Code 001), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Brasília, DF; Grant 420913/2018-1). We also thank CNPq for granting a scholarship to MM (DCR-314905/2018-9). We would like to thank Editage (www.editage.com) for English language editing.
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This work was supported by Fundação de Amparo à Pesquisa do Estado de Mato Grosso (FAPEMAT) (Cuiabá, MT), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (Belo Horizonte, MG; Grant APQ-00772-19), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Brasília, DF; Finance Code 001), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Brasília, DF; Grant 420913/2018-1).
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MLS, AIM, and DIR designed the study; AIM and NTS established organogenic cultures; AIM, LASS, and LCSB assisted with histological evaluations; MM and NTS performed biochemical analyses; ACR, EMM, and LFV performed cytogenetics and flow cytometry analyses; NTS, CSS, MLS, and MDM performed morphometric evaluations; AIM, DIR, MLS, and WCO wrote the manuscript; MM, IFC, and LFV revised the final version of the manuscript.
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Mikovski, A.I., da Silva, N.T., Silva, L.A.S. et al. From endosperm to triploid plants: a stepwise characterization of the de novo shoot organogenesis and morpho-agronomic aspects of an ornamental passion fruit (Passiflora foetida L.). Plant Cell Tiss Organ Cult 147, 239–253 (2021). https://doi.org/10.1007/s11240-021-02120-4
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DOI: https://doi.org/10.1007/s11240-021-02120-4