Abstract
In woody plants, the availability of plant material for in vitro use is usually limited to the short active growth period of the mother plants. To overcome this limitation, an effective protocol is described to break the seasonal effect on in vitro axillary bud sprouting in Leucospermum cordifolium ‘Flame Spike’ and Leucospermum ‘Tango’. Shoots were harvested at four different times of the year, sterilized and cultured in forcing solutions containing a quarter-strength of Murashige and Skoog medium salts (MS, 1962), supplemented with benzyladenine (BA) (0–100 mg L−1) and gibberellic acid (GA3) (0 or 10 mg L−1). After 10 days, multinodal explants were cultured on half-strength MS solid growth regulator-free medium or with GA3. The effect of pre-treatment is clear in the case of L. cordifolium ‘Flame Spike’ and although in L. ‘Tango’ was not evident, its use reduced the time required for in vitro establishment.
Key message
The use of nutritive solutions with and without growth regulators allowed to increase the in vitro axillary buds sprouting and the percentage of new shoots
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ES and JFPF planned and designed the research, JARP contributed plant material, ES performed the experiments and collected data. ES, JFPF and JARP analysed the data, ES wrote the manuscript, CA assist with writing and ES, CA, JFPF, JARP supervised the writing. All the co-authors reviewed the manuscript before submission.
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Communicated by M. I. Beruto.
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Suárez, E., Alfayate, C., Rodríguez-Pérez, J.A. et al. Effect of forcing solutions used to break the seasonal influence on in vitro axillary bud sprouting of two Leucospermum (R. Br.) cultivars. Plant Cell Tiss Organ Cult 147, 175–180 (2021). https://doi.org/10.1007/s11240-021-02099-y
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DOI: https://doi.org/10.1007/s11240-021-02099-y