Abstract
Hairy root cultures are often used to produce valuable metabolites. They are grown on sucrose-rich medium, which is highly susceptible to contamination. Trapa natans is a unique plant with photosynthesizing roots. It is a promising object to obtain photoautotrophic hairy root culture. Protocols for transformation of this species are yet unknown. We report that hairy roots can be induced in aquarium and in vitro cultures of T. natans by agrobacterium-mediated and biolistic transformation. 64 roots were induced by Agrobacterium rhizogenes strain 15834, two roots were obtained using strain K599. Strain A4 was not effective. Biolistics with either amplicons of rol genes and 1301 pCAMBIA plasmid carrying rol genes resulted in the formation of six roots. All these roots contained chloroplasts. This achievement opens a prospect for genetic transformation of T. natans and use of its green photosynthesizing hairy root cultures in production of bioactive substances and in phytoremediation.
Key message
Protocols for the Agrobacterium-mediated and biolistic transformation of Trapa natans to induce hairy roots have been developed for the first time. All induced roots contained chloroplasts.
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Data availability
Data sharing not applicable to this article as no datasets were generated or analysed during the current study.
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This study was funded by Russian Science Foundation, project No 18-74-00056.
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EVM designed the experiments and wrote the manuscript, AA, KhM and MP obtained and transformed aquarium and in vitro cultures, GG performed biolistic transformation, BK supervised the project. All authors read and approved the final manuscript.
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Communicated by Amita Bhattacharya.
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Mikhaylova, E.V., Artyukhin, A., Musin, K. et al. The first report on the induction of hairy roots in Trapa natans, a unique aquatic plant with photosynthesizing roots. Plant Cell Tiss Organ Cult 144, 485–490 (2021). https://doi.org/10.1007/s11240-020-01963-7
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DOI: https://doi.org/10.1007/s11240-020-01963-7