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Cloning and functional identification of a strigolactone receptor gene MdD14 in apple

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Abstract

Strigolactones (SLs) are phytohomones that regulate shoot branching and hypocotyl elongation. Here, the strigolactone receptor gene MdD14 in apple was cloned. MdD14 was localized to the nucleus and cytoplasm. GUS staining showed that MdD14 was expressed in various tissues. A promoter analysis revealed that MdD14 contained multiple response elements and its expression levels were induced by various abiotic stress treatment. The post-translational of MdD14 was regulated by the SL analog GR24. Ectopic expression of MdD14 in Arabidopsis is resulted in a phenotype that inhibited shoot branching and hypocotyl elongation, as well as increased tolerance to salt, drought and low temperature stresses. These findings reveal the functions of MdD14 and lay a foundation for studying SLs in apple.

Key message

The apple MdD14 plays a key role in the SL signal transduction pathway and is involved in various abiotic stress resistance.

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Acknowledgements

This work was supported by NSFC (31430074), Major Program of Shandong Provincial Natural Science Foundation (ZR2017ZC0328), Shandong Province Government (SDAIT-06-03), and Ministry of Agriculture of China (CARS-27).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, College of Horticulture Science and Engineering, Shandong Agricultural University, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Tai’an, 271018, Shandong, China

    Yu-Ying Yang, Yi-Ran Ren, Peng-Fei Zheng, Ling-Ling Zhao, Chun-Xiang You, Xiao-Fei Wang & Yu-Jin Hao

  2. Yantai Academy of Agricultural Sciences, Yan-Tai, 265599, Shandong, China

    Yu-Ying Yang, Xiao-Fei Wang & Yu-Jin Hao

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  1. Yu-Ying Yang
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  4. Ling-Ling Zhao
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Contributions

Yu-Jin Hao, Xiao-Fei Wang, and Yu-Ying Yang conceived and designed the experiments; Xiao-Fei Wang and Yu-Jin Hao supervised the experiments; Yi-Ran Ren and Peng-Fei Zheng performed most of the experiments; Yu-Jin Hao and Yu-Ying Yang conceived the project and wrote the article with contributions from all the authors.

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Correspondence to Xiao-Fei Wang or Yu-Jin Hao.

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Communicated by Henryk Flachowsky.

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Figure S1

Acquisition of transgenic plants. (A) GUS staining of ProMdD14::GUS transgenic Arabidopsis. (B) GUS staining of ProMdD14::GUS transgenic calli. (C) The expression of MdD14 by RT-qPCR in MdD14-OE transgenic calli and MdD14-anti transgenic calli. (D) Electrophoresis of PCR products for cloning of MdD14-OE transgenic Arabidopsis. (E) The expression of MdD14 by RT-qPCR in MdD14-OE transgenic Arabidopsis. Supplementary material 1 (PNG 1315 kb)

Figure S2

Response of MdD14 transgenic calli to salt, drought and low temperature. (A) The phenotype of MdD14-OE transgenic calli treated with NaCl and PEG. The fresh height (B), MDA (C), and relative electrical conductivity (D) of Figure S2A. (E) The phenotype of MdD14-OE transgenic calli treated with different temperture. The fresh height (F), MDA (G), and relative electrical conductivity (H) of Figure S2E. Supplementary material 2 (JPEG 3231 kb)

Table S1

GenBank accession numbers of D14 orthologs used in Fig. 1. Supplementary material 3 (PNG 2203 kb)

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Yang, YY., Ren, YR., Zheng, PF. et al. Cloning and functional identification of a strigolactone receptor gene MdD14 in apple. Plant Cell Tiss Organ Cult 140, 197–208 (2020). https://doi.org/10.1007/s11240-019-01722-3

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  • DOI: https://doi.org/10.1007/s11240-019-01722-3

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