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Haploid plant regeneration from hardy kiwifruit (Actinidia arguta Planch.) anther culture

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Abstract

Haploids are important for studying plant breeding and genome sequencing. Regeneration from in vitro anther culture under aseptic conditions has achieved success in many higher plants. This study focused on anther culture of hardy kiwifruit (Actinidia arguta Planch.) to obtain haploid or double haploid plants that originated from microspores. Using the anthers of hardy kiwifruit as the test materials, we report the relationship between the microspore development stage and the bud morphological characteristics, some factors influenced the callus induction and the differentiation of the adventitious buds. The results showed that microspore development correlated with the bud diameter and morphological characteristics during development. The microspore development stage, pretreatment at low temperature (4 °C), basic medium, plant growth regulators and genotype all affect hardy kiwifruit anther callus induction, and the type and concentration of plant growth regulators significantly affect the regeneration of adventitious buds. The ploidy of 119 regenerated plants was verified using flow cytometry. The results showed that the ploidy of the plantlets obtained from the vitro anther culture of hardy kiwifruit was complex, being 3.36% haploid, 56.3% tetraploid, 4.20% hexaploid, and 36.13% octoploid.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

6-BA:

6-Benzylaminopurine

KT:

Kinetin

NAA:

α-Naphthaleneacetic acid

IBA:

Indole-3-butyric acid

ZT:

Zeatin

TDZ:

Thidiazuron

2-ip:

z-IP

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Acknowledgements

This research was supported by the Project of Science and Technology Department of Jilin Province (#20170203006NY) and the Central Public-interest Scientific Institution Basal Research Fund (#1610342016019).

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    Authors and Affiliations

    1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China

      Guang-Fu Wang, Hong-Yan Qin, Dan Sun, Shu-Tian Fan, Yi-Ming Yang, Zhen-Xing Wang, Pei-Llei Xu, Ying Zhao, Ying-Xue Liu & Jun Ai

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    1. Guang-Fu Wang
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    2. Hong-Yan Qin
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    3. Dan Sun
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    Contributions

    Designed the experiments: HYQ and JA. Performed the experiments: GFW. Analysed the data: DS. Contributed reagents/materials/analysis tools: STF, YMY and ZXW. Checked and corrected the manuscript: PLX, YZ and YXL. Wrote the paper: GFW and HYQ. All co-authors agree on the contents of the final manuscript and approve it.

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    Correspondence to Jun Ai.

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    The authors declare that they have no conflict of interest.

    Additional information

    Communicated by Sergio J. Ochatt.

    Guang-Fu Wang and Hong-Yan Qin contributed equally to this study.

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    Wang, GF., Qin, HY., Sun, D. et al. Haploid plant regeneration from hardy kiwifruit (Actinidia arguta Planch.) anther culture. Plant Cell Tiss Organ Cult 134, 15–28 (2018). https://doi.org/10.1007/s11240-018-1396-7

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    • DOI: https://doi.org/10.1007/s11240-018-1396-7

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